LOCUS       sequence-76143-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with 4 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-4XDSR
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8..41)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 caagcttata acttcgtata gcatacatta tacgaagtta ttgctagccg ttttcttcaa
       61 ttactaaatt agaactaatt caatcatatg ctggcatgcg gagagctcgg agtcgacgag
      121 actagtactg gttctacagg atcaaccggc tccacggggt cgatgggtag atctatgtct
      181 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
      241 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
      301 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
      361 actttaactt atggtgttca atgtttttct agatacccag atcacatgaa acaacatgac
      421 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
      481 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
      541 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
      601 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
      661 gttaacttca aaatta
//
