LOCUS       sequence-76149-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal 3XHA tagged gene 
            sequences together with 4 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-c3HA-4XDSR
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             174..263
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(361..378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(361..377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(370..392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aagcttataa cttcgtatag catacattat acgaagttat tgctagccgt tttcttcaat
       61 tactaaatta gaactaattc aatcatatgc tggcatgcgg agagctcgga gtcgacgaga
      121 ctagtactgg ttctacagga tcaaccggct ccacggggtc gatgggtaga tcttacccat
      181 acgatgttcc tgactatgcg ggctatccgt atgacgtccc ggactatgca ggatcctatc
      241 catacgacgt tccagattac gcttaaagat ctttaaactc cgtattaaac ccattcttaa
      301 acagaacttt aaaccccggg ataacttcgt atatggtatt atatacgaag ttatgaattc
      361 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      421 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      481 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      541 tacgcatctg tgcggtattt cacaccgcat accatcgtcc ggatcaatga agtagatgag
      601 tttactacct gtatatactt ttttttttgt c
//
