LOCUS       sequence-76201-e        7545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PF10_0166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PF10_0166-bio-His
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2709..2726
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2778..2833
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2832..2851)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5069..5087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5155..5259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5260..5328,5329..6120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5478..5497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6291..6879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6780..6799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7033..7050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7167..7188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7203..7208,7209..7226,7227..7233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7241..7257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7246..7268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7265..7281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7265..7281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 tgaaaaccca gaagaacgag ggcatcatca tcgacaaaga gggcaaagag aacgacaacg
     1261 gcaacagcga ccggaacacc cccaacaaca acaatcggct gaaccggatg accctgaaca
     1321 aagagatgta cgagaagcac aacaagatga acaacgaaga ggataacaac aagaacatca
     1381 actacgacct gagcaacctg aaggacggca tgaagaagaa gcacttcggc agcaacagcg
     1441 ccgaggacaa gaccaacaag aagtccagca tcaacagcgt gctcgagaag taccagcaca
     1501 ccaagcacga ggaaaagaac ctgcagaacc tgaacaagaa tatgatgacc cacgagaacg
     1561 acgacagcat gaagtccatc ttcatcagcg aggaaagctt cgagagcgcc aagaacgtgt
     1621 gccagttcat cgtgatgcag aacgactact tcaagaactt ctgcaatatc gccagcctgg
     1681 tgtacgacat cgagaagctc aagaagaaca accacaagat ctacgaggaa atcctgtcca
     1741 acagctacga gaacaacaat ggcgccaagt tcaagctgct gaaagagatc aaccacgcca
     1801 aggtgttcaa agaaatggac aacaccaacc ccaagttcag catcctgttc ctctacgaca
     1861 agttctgcta cggcggcatc cccctggcct gctccaacct gatgaagtac gacaacgtgt
     1921 tcgacatcaa cgagcagagc gaaaccacca acaagcccga cgacaatgtg aacatggaag
     1981 agatggaaaa ctacatcatc aaccaggccg gcatggaaaa cggcttcgag gaaaacgtgg
     2041 ccgacctgac ccaggaaggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2101 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2161 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2221 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2281 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2341 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2401 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2461 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2521 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2581 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     2641 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     2701 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     2761 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2821 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     2881 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2941 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3001 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3061 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3121 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3181 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3241 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3301 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3361 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3421 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3481 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3541 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3601 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3661 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3721 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3781 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3841 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3901 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3961 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4021 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4081 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4141 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4201 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4261 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4321 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4381 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4441 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4501 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4561 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4621 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4681 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4741 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4801 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4861 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4921 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4981 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5041 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5101 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5461 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7081 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7141 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7201 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7261 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7321 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7381 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     7441 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     7501 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
