LOCUS       sequence-76203-e        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSP3.5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSP3.5-bio-His
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3924..3941
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3993..4048
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4047..4066)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6284..6302)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6370..6474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6475..6543,6544..7335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6693..6712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7506..8094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7995..8014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8248..8265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8382..8403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8418..8423,8424..8441,8442..8448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8456..8472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8461..8483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8480..8496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8480..8496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 ccagcatcag ctgcgagatc gattacaccc ccagcaccaa tatcgccagc aacctgaact
     1261 ccaacctgcg gagctgcagc agcaatctgg tgctgagcag caacatcgac agcaccaagc
     1321 tggaaggcac ctacggcgag aacctgaacg acgccgtgtc cctgaccaac aacatcaacg
     1381 accacaccac caacgaggcc aacattgcca acgtggcaaa tatcgcgaac gaggcgaata
     1441 ttgctaatga ggcctccatc accaatcagg ccaacctggc cagcgagact aatatcgcct
     1501 ccgagacaaa cattgctaac gaagccagcg tgcccaatga ggccagcgtg aacgaggtgc
     1561 cccagctgga agtgcccaag gacgccgtgg aaaaccacgc cgagagcaag gacgtgctgc
     1621 tgaacgagaa agagaacttc gccaacggcg tggaaacaca cgtggacctg ggcagccaag
     1681 agcagtactt cggcagcagc tacgacatga acatggacac cgagggcggc atcaagaaat
     1741 tcaagaacgt gttccagagc tacttcaacc aggccaaggg caacagcggc acagagggcg
     1801 acggaagctc cgtgtttggc agcatcttcg gctccctgct gacccccatc gatagcctgc
     1861 tggaaaagtt catcggcagc aacaacgcca acagcgactc caacgtgaag aacaccgcca
     1921 tgggcaacgg ccagaacaaa tacgacaaca atatctacct ggacgaagag gacgccctgt
     1981 ccgacgccga gcactacaac gatggctcca tcagcctggg cgaggaagat gagctgagcg
     2041 acgccgaaca ttataacgac ggcagcattt ccctcgatga agaggatgag ctgtctgatg
     2101 cggagcacta taatgacggc ggcatctgcc ttggagaaga agatgaactc tctgacgctg
     2161 aacattacaa tgatggcgga atcagcctcg atgaggaaga tgtgctctcc gacgcagagc
     2221 actataatga tggggacatc agcctggacg aagatgagct gtccgatacc gagaactact
     2281 acgacggcgg aatctccctg gatgagagcg acgacctgag cgaccctgag agcaagacca
     2341 aagaggacaa ctaccacctg tactactggg acgacttcta ccacgagtac aagcccacct
     2401 acctgaatta ccacatgcac tacaccctgt acgagcccaa caacttctac gataccacca
     2461 atgaggaaac ccacaatttc tacaacacgg ccaacgaaga gagccacaac ttttacaacc
     2521 ccgcccacga ggaatcgcat aacttctata atcctgccca cgaagagtcg cacaattttt
     2581 acaatccggc gcatgaagag tcccataatt tctataaccc ggctcacgag gaaagtcaca
     2641 acttctacaa tccagctcat gaggaaagcc ataacttcta taaccccgcg catgaagaga
     2701 gtcacaattt ctataatccc gcgcacgaag aatcacacaa cttctacaac ccggcacatg
     2761 aagaatctca taacttttac aacccagctc acgaagaaag ccacaatttc tacaaccctg
     2821 ctcacgaaga gtcccacaac ttctataatc cagcccatga agaatcacat aatttctaca
     2881 cccccacgca cgaggaatct cacaattttt acaatcctgc acacgaggaa tcacacaatt
     2941 tctataaccc cgcccatgag gaaagccaca atttttacaa tccagcccac gaagagagtc
     3001 ataacttcta taaccctgca cacgaggaaa gccataactt ttacaatcct gcacatgaag
     3061 agagccacaa cttctatacc cctacccacg acgagttcaa cgtgcccctg aactacaatc
     3121 acgactacga ctacaattac ttcgagaacg acaactacaa catccagaac gtgaaggaca
     3181 acctggtcaa gaaagtgaac gacttcatgg aaagcgacaa cctgctggtc aacaccttca
     3241 agggcattgc cggcggagtg accagcttct ttggcgcgcc gtcgacctcc atcacggcct
     3301 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     3361 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     3421 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     3481 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     3541 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     3601 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     3661 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     3721 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     3781 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     3841 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     3901 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     3961 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     4021 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     4081 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     4141 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     4201 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     4261 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     4321 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     4381 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     4441 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     4501 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     4561 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     4621 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     4681 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     4741 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     4801 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     4861 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     4921 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     4981 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     5041 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     5101 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     5161 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     5221 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     5281 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     5341 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     5401 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     5461 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     5521 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     5581 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     5641 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     5701 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5761 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     5821 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5881 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     5941 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     6001 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     6061 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     6121 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     6181 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     6241 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     6301 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6361 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6421 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6481 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6541 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6601 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6661 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6721 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6781 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6841 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6901 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6961 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     7021 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7081 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7141 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7201 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7261 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7321 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7381 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7441 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7501 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7561 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7621 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7681 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7741 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7801 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7861 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7921 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7981 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8041 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8101 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8161 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8221 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8281 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8341 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8401 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8461 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     8521 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     8581 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     8641 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     8701 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
