LOCUS       sequence-76231-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA under control of the Drosophila U6:2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD2-dU6:2gRNA
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(284..359)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(378..777)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 gttggttggc acaccacaaa tatactgttg ccgagcacaa ttgtctagaa tgcatacgca
       61 ttaagcgaac attaaaaaga tgtgaaaaca taactattat gtctaaataa acacacgtca
      121 gatgtatgta cgtcaacgga aaaccattgt ctatatatta caattactaa atacatacca
      181 aattgaatac atattgatga aaaataataa atactggcga aagcaaaaaa acgaaacatt
      241 tttattttat tgaacaactc tcaggctcca ggtaggcaaa aaagcaccga ctcggtgcca
      301 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
      361 ggtcttctcg aagacccgaa gtattgagga aaacatacct atataaatga tcaacatcag
      421 gaaagagcag ttgagaatta taagaattgg caaatggtct taagaaccct ctgcttaaga
      481 ttttcaaaat ttcctttaga atcaaagtgt cctattgttc gtttgttgaa aacacagttc
      541 gatttattga ctataataaa ttgatagttt taaatataga ggcacgacta agagagtttg
      601 gttttgtttt ggttagggac caaaaaaagt atacataacg aataaaaaag gatttaagtg
      661 caaatggtaa aaaagtgtcg agtttttctt gagttgattg tgctgtaatg agactctgca
      721 ttcggcattt gactcggctt ttcctactcg tgccgtattt caggctgcaa gtcgaacgaa
      781 ttcagatctc aattgatcgg ctaaatggta tggcaagaaa aggtatgcaa tataataatc
      841 ttttattggg tatgcaacga aaatttgttt cgtcaacgta tg
//
