LOCUS       sequence-76237-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses VSVG fused to UVR8 for light-triggered trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: VSVG-YFP-2xUVR8
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           94..133
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac
      121 ctcttcggcc agcgaattca agcttcgaat tcgccatgaa gtgccttttg tacttagctt
      181 ttttattcat cggggtgaat tgcaagttca ccatagtttt tccacacaac caaaaaggaa
      241 actggaaaaa tgttccttcc aattaccatt attgcccgtc aagctcagat ttaaattggc
      301 ataatgactt aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc
      361 aagcagacgg ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt
      421 acggaccgaa gtatataaca cattccatcc gatccttcac tccatctgta gaacaatgca
      481 aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa
      541 gttgtggata tgcaactgtg acggatgctg aagcagcgat tgtccaggtg actcctcacc
      601 atgtgcttgt tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat
      661 gcagcaatga catatgcccc actgtccata actccacaac ctggcattcc gactataagg
      721 tcaaagggct atgtgattct aacctcattt cca
//
