LOCUS       sequence-7624-ex       11128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 11128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pBAC-BA-lacZ
FEATURES             Location/Qualifiers
     source          1..11128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..127
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      219..246
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     324..342
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(413..418,419..436,437..443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     453..475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     472..488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     472..488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(525..542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(525..541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             532..3579
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(534..556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    3646..3679
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3697..4095
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    4355..4828
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(4901..5872)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(5872..7047)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7373..7623
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7626..8381)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8472..8691
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      8759..9290
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8767..9381
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        10239..10341
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10342..11001
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10408..10427)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcggccgcaa cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
       61 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      121 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
      181 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
      241 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
      301 cgctcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
      361 tctgtggaag atcttcgaca cgcagaagcc cattacatta ggcaccccgg gctttacact
      421 ttatgcttcc ggctcgtatg ttgtgtcgac cgagcggata acaatttcac acaggaaaca
      481 gctatgacca tgattacgaa tttcgacctg cagccaagct tggcactggc cgtcgtttta
      541 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      601 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      661 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
      721 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
      781 atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg
      841 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
      901 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
      961 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
     1021 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg
     1081 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
     1141 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
     1201 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
     1261 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
     1321 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
     1381 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
     1441 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt
     1501 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt
     1561 cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg
     1621 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat
     1681 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat
     1741 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc
     1801 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg
     1861 agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg
     1921 tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc
     1981 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc
     2041 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc
     2101 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa
     2161 tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg
     2221 gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat
     2281 tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc
     2341 acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta
     2401 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc
     2461 ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat
     2521 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc
     2581 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc
     2641 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc
     2701 gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg
     2761 ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc
     2821 gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac
     2881 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag
     2941 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct
     3001 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc
     3061 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
     3121 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta
     3181 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
     3241 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc
     3301 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg
     3361 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc
     3421 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg
     3481 gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca gctgagcgcc
     3541 ggtcgctacc attaccagtt ggtctggtgt caaaaataat taattaactc cggatctaga
     3601 gcccgcctaa tgagcgggct tttttttctt aaggccgcat cgaatataac ttcgtataat
     3661 gtatgctata cgaagttatt agcgatgagc tcggacttcc attgttcatt ccacggacaa
     3721 aaacagagaa aggaaacgac agaggccaaa aagctcgctt tcagcacctg tcgtttcctt
     3781 tcttttcaga gggtatttta aataaaaaca ttaagttatg acgaagaaga acggaaacgc
     3841 cttaaaccgg aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg
     3901 tcggatcacc ggaaaggacc cgtaaagtga taatgattat catctacata tcacaacgtg
     3961 cgtggaggcc atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga
     4021 tctgcatcaa cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaatacg
     4081 gggcaacctc atgtccgagc tcgcgagctc gtcgacagcg acacacttgc atcggatgca
     4141 gcccggttaa cgtgccggca cggcctgggt aaccaggtat tttgtccaca taaccgtgcg
     4201 caaaatgttg tggataagca ggacacagca gcaatccaca gcaggcatac aaccgcacac
     4261 cgaggttact ccgttctaca ggttacgacg acatgtcaat acttgccctt gacaggcatt
     4321 gatggaatcg tagtctcacg ctgatagtct gatcgacaat acaagtggga ccgtggtccc
     4381 agaccgataa tcagaccgac aacacgagtg ggatcgtggt cccagactaa taatcagacc
     4441 gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac
     4501 cgtggttcca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
     4561 aatcagaccg acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac
     4621 gagtgggacc gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc
     4681 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac
     4741 cgacgatacg agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa
     4801 cagtgggccc agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat
     4861 taagtaaaga cagataaacg tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa
     4921 gttccttaag aatggcctca attttctcta tacactcagt tggaacacgg gacctgtcca
     4981 ggttaagcac cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc
     5041 gtgagcttaa actagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga
     5101 taacttcttc agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct
     5161 gctgcttaag taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg
     5221 cagatagttc accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt
     5281 tgatacagcg ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat
     5341 ttccagcaaa ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg
     5401 ttgggcgata atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc
     5461 caaccagaac acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat
     5521 cggcaatttc tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca
     5581 gtagaaaaga agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt
     5641 cattacctga ccatacccga gaggtcttct caacactatc accccggagc acttcaagag
     5701 taaacttcac atcccgacca catacaggca aagtaatggc attaccgcga gccattactc
     5761 ctacgcgcgc aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt
     5821 caaccggttg agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat
     5881 ctcccagcgt ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga
     5941 aagagcattt ctccaggcac cagttgaaga gcgttgatca atggcctgtt caaaaacagt
     6001 tctcatccgg atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac
     6061 catgcttccc caggcatccc gaatttgctc ctccatccac ggggactgag agccattact
     6121 attgctgtat ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt
     6181 cttgagcaga tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc
     6241 agcaggcgtg ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc
     6301 caggttaggc gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc
     6361 cagtcggagc atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa
     6421 ctcagtttca atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca
     6481 agtgggcttt attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt
     6541 gtcttctgca tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg
     6601 ggggtcgtta ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag
     6661 atgaacagaa actgaggttt tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg
     6721 tggaaatacg tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat
     6781 ttgttcaatt gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg
     6841 cggtagtcgc cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc
     6901 cgctgcttca cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt
     6961 aaactgtgca atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag
     7021 tgtttccatg agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa
     7081 tcttgcaatt tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt
     7141 gtttaaaata agagcaacac tacaaaagga gataagaaga gcacatacct cagtcactta
     7201 ttatcactag cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag
     7261 caaagaagaa ctgttctgtc agatagctct tacgctcagc gcaagaagaa atatccaccg
     7321 tgggaaaaac tccaggtaga ggtacacacg cggatagcca attcagagta ataaactgtg
     7381 ataatcaacc ctcatcaatg atgacgaact aacccccgat atcaggtcac atgacgaagg
     7441 gaaagagaag gaaatcaact gtgacaaact gccctcaaat ttggcttcct taaaaattac
     7501 agttcaaaaa gtatgagaaa atccatgcag gctgaaggaa acagcaaaac tgtgacaaat
     7561 taccctcagt aggtcagaac aaatgtgacg aaccaccctc aaatctgtga cagataaccc
     7621 tcagactatc ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc
     7681 tggcggcctt tctttttctc aatgtatgag aggcgcattg gagttctgct gttgatctca
     7741 ttaacacaga cctgcaggaa gcggcggcgg aagtcaggca tacgctggta actttgaggc
     7801 agctggtaac gctctatgat ccagtcgatt ttcagagaga cgatgcctga gccatccggc
     7861 ttacgatact gacacaggga ttcgtataaa cgcatggcat acggattggt gatttctttt
     7921 gtttcactaa gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga
     7981 tatgggttga tatgtacact gtaaagccct ctggatggac tgtgcgcacg tttgataaac
     8041 caaggaaaag attcatagcc tttttcatcg ccggcatcct cttcagggcg ataaaaaacc
     8101 acttccttcc ccgcgaaact cttcaatgcc tgccgtatat ccttactggc ttccgcagag
     8161 gtcaatccga atatttcagc atatttagca acatggatct cgcagatacc gtcatgttcc
     8221 tgtagggtgc catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga
     8281 tcccgggaga gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta
     8341 tttttacgtt tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg
     8401 tgacaagttt ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg
     8461 tgaaaaaaca gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga
     8521 caggactgtc atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc
     8581 tctagaacca gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta
     8641 taaaaaaaat aattataaaa atatccccgt ggataagtgg ataaccccaa gggaagtttt
     8701 ttcaggcatc gtgtgtaagc agaatatata agtgctgttc cctggtgctt cctcgctcac
     8761 tcgaccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac
     8821 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag
     8881 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct
     8941 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca
     9001 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc
     9061 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg
     9121 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc
     9181 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat
     9241 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc ggccgcggtg
     9301 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
     9361 cccagcggcg agggcaacca gccgagggct tcgccctgtc gctcgactgc ggcgagcact
     9421 actggctgta aaaggacaga ccacatcatg gttctgtgtt cattaggttg ttctgtccat
     9481 tgctgacata atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga
     9541 aggcatattc aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct
     9601 ataaacgcta cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt
     9661 tcccgactgt ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa
     9721 aaacagcata tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg
     9781 ataattgttt cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc
     9841 gggcagccac atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat
     9901 gtcccatcag gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat
     9961 aggaatggcg gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag
    10021 cggcggcaac cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc
    10081 gcgctttctc tgtccttcct gtgcgacggt tacgccgctc catgagctta tcgcgaataa
    10141 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc
    10201 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc
    10261 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
    10321 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
    10381 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
    10441 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
    10501 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt
    10561 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
    10621 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
    10681 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
    10741 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
    10801 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
    10861 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
    10921 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
    10981 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
    11041 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt
    11101 caaacatgag aattggtcga cggcccgg
//
