LOCUS       sequence-76289-e       10654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic PI3K activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR iSH-YFP-PIF
FEATURES             Location/Qualifiers
     source          1..10654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    177..409
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             594..638
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    923..1040
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1193..1600
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1634..1643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2306..2308,2309..2311,2312..3022)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2351..2372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2612..2631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2959..2980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3391..3979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3444..3464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3862..3873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3982..3998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3983..3999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4089..4322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4347..4365)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4348..4365)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4655..4674)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4888..4907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5007..5029
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5067..5085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5153..5257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5258..5326,5327..6118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5476..5495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6289..6877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6778..6797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7031..7048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7118..7138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7123..7452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7303..7438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8586..8651
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8781..8801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9226..9360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9263..9282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9317..9336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9529..10162
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10209..10334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
       61 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
      121 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
      181 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
      241 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
      301 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
      361 caggcaagaa tcctggctgt gaaagatacc taaaggatca acagctcctg gggatttggg
      421 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
      481 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
      541 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
      601 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
      661 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
      721 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
      781 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
      841 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
      901 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      961 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1021 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg
     1081 ataagcttga tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca
     1141 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc
     1201 attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg
     1261 tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc
     1321 ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca
     1381 gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca
     1441 ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct
     1501 tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc
     1561 actcggcgcg ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag
     1621 aattctcacg cgtgccacca tggcatccaa gtaccaacaa gaccaggtgg tgaaggagga
     1681 cagcgtagag gctgtgggcg cccagctcaa ggtctaccac cagcagtacc aggacaagag
     1741 ccgcgaatat gaccagctgt atgaagaata cacacggacc tcccaggagc tgcagatgaa
     1801 gcgcacagcc atagaggcct tcaacgagac catcaagatc ttcgaagagc agggccagac
     1861 acaggagaag tgcagcaagg agtatttgga gcgcttccgg cgagagggaa atgagaagga
     1921 gatgcagagg atcctgctga actccgagcg actcaagtct cgcatcgcgg agatacacga
     1981 aagccgcacg aagttggagc aggatctgcg ggcgcaggcc tccgacaacc gtgagatcga
     2041 caagcgcatg aacagcctca aacctgacct catgcagctg cgcaagatca gggaccagta
     2101 cctcgtgtgg ctcacccaga aaggtgcccg acagaggaag atcaacgaat ggctgggaat
     2161 caagaacgag actgaggacc agtattcact gatggaggat gaggacgccc tcccccacca
     2221 cgaggagcgc acggaattca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     2281 tggtggtagt gctggtggtg ctagcatggt gagcaagggc gaggagctgt tcaccggggt
     2341 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2401 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2461 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     2521 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2581 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2641 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2701 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2761 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2821 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2881 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2941 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3001 cggcatggac gagctgtaca agaccggtat gatgttctta ccaaccgatt attgttgcag
     3061 gttaagcgat caagagtata tggagcttgt gtttgagaat ggccagattc ttgcaaaggg
     3121 ccaaagatcc aacgtttctc tgcataatca acgtaccaaa tcgatcatgg atttgtatga
     3181 ggcagagtat aacgaggatt tcatgaagag tatcatccat ggtggtggtg gtgccatcac
     3241 aaatctcggg gacacgcagg ttgttccaca aagtcatgtt gctgctgccc atgaaacaaa
     3301 catgttggaa agcaataaac atgttgacta agcggccgcg actctagagt cgacctgcag
     3361 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3781 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3901 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3961 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
     4021 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4081 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4321 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     4381 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     4441 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     4501 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     4561 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     4621 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     4681 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     4741 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     4801 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     4861 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4921 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4981 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5041 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5101 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5161 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6421 gataccaaat actgtctttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6901 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6961 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7021 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7081 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     7141 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7201 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7261 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7321 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7381 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7441 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     7501 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     7561 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     7621 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     7681 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     7741 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     7801 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     7861 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     7921 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     7981 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     8041 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     8101 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     8161 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     8221 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     8281 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     8341 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     8401 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     8461 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     8521 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     8581 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     8641 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     8701 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     8761 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     8821 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     8881 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     8941 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     9001 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     9061 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     9121 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9181 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9361 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9421 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9481 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     9541 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9601 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     9661 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     9721 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     9781 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     9841 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     9901 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     9961 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10021 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10081 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10141 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10201 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10261 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10321 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10381 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10441 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10501 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10561 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10621 tagacaagat agaggaagag caaaacaaaa gtaa
//
