LOCUS       sequence-76301-e        9669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA1-bio-His
FEATURES             Location/Qualifiers
     source          1..9669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4833..4850
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4902..4957
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4956..4975)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7193..7211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7279..7383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7384..7452,7453..8244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7602..7621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8415..9003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8904..8923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9157..9174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9291..9312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9327..9332,9333..9350,9351..9357)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9365..9381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9370..9392
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9389..9405
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9389..9405
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 tgaagttctt catcccctac gtgtacatca tctgcgtgat cttcatcatc aacgtgatcc
     1261 ggaccagagg cgaagaggac gacgataaca acaatatcgc cggcaagagc atcctgggca
     1321 ccagccacaa caacattgcc aacatcgacc tgagcagcat ccccaacctg gacagcaaca
     1381 tccacgccag cttcagcagc gacaccaaag agtggtcccc caacaacctg gcctccaaga
     1441 agaaaaagaa gaagaaagag atccggccca aggacatcat gagcaacagc gacagcagca
     1501 acaccgcctc catcaacaag cagaacaaca accagatcaa gagcgtgctg ctgaaagaga
     1561 acaagggcgt caagatcacc ggcccctgca acgtgaacct ggccatcttc ctggtgcccc
     1621 acatctacat cgacgtggaa acaaagtata acaatattga gctgaagtac gagctggacg
     1681 agttcagcga ctccatcaag ttcaaggaca ccaccaccga gctgcggacc agcgacgaca
     1741 ccctgatgaa caccaacttc aacgtgggcg tgtccagaga caccctggac aaggaccggc
     1801 tgtacaatat ctgcgccgag aacaaagcct tcaagttcgt ggtctacatc aaggacaaca
     1861 tcctgaccct gaagtggaag gtgtacgaga caggcgtgac caacaacaag gtggacatcc
     1921 ggcagtacaa gatgaaggaa ctgacccggc ccatcaccac catccagatc cacagcgtgt
     1981 ccgaaaacaa ggatacccat ctgctcgaga gcaagaacta cgtgatcaag accgacatcc
     2041 ccgagacatg cgacgtgatg gccaccaact gctttctgag cggcaacatc aacatcgaga
     2101 agtgcctgga atgcaccctg ctggtgcaga acaatgacgc cagcagcgag tgcttcacct
     2161 acgtgtccaa cgacgtgcgc gagaacttca atcagatcaa ggccgaggcc gaggacgatg
     2221 agaacttccg gaactaccac ctgaccgaca caatcaacaa tattctgaag cggatctaca
     2281 agatcaacaa gaacgagggc aagaaagagc tgatcaccct ggaagaactg gacaacttcc
     2341 tcaaagagag catcaccgac tactgcaaga tcctgagaga gatcgacacc aacggcgccc
     2401 tggtcaacca cgagctgggc aacaatgtgg acgtgttcaa caatctgatc cggctgctca
     2461 agctgcacaa gaatgaggcc atcagcaccc tgcacaacaa gctgagaaac agcgccatct
     2521 gcatgaagta ccccgacaag tggatcgaga agaaaaccgg cctgatcctg cccaacgtgg
     2581 tcaacaacaa catcatctac aacaacaaat acgagaagct gaacgaggaa aagaagcgga
     2641 agatctacga caacaaggac gacagcaaga tcagcgacat catcaatatc aagaagtaca
     2701 tcttcaccaa caacgccctc aagtacttca acaacgacaa gcagttctgc aactccgcct
     2761 tctgcaaccg gctgaaggac gagaacaact gcatctccaa gatccagatc gaggaccagg
     2821 gcaactgcgc catcagctgg atcttcgcca gcaagtacta cctggaaacc ctcaagtgca
     2881 tgaagggcta cgagccccac gccatctccg ccctgtatat cgccaattgc gccaagcgga
     2941 agcacaagaa ccggtgcaat gtgggcagca accccctgga attcctgcag attatcgagg
     3001 aaaaccagtt cctgcccatg gacaccaact acctgtactc ctacaccaaa gtgggcaacg
     3061 actgccccga cgaagagaag aattgggtca acctgctgaa acacacccgg atgctgaact
     3121 ataacaacaa gcacagaagc accctgagca ccaaggccta ccgggcctac gagagcgagc
     3181 acttcaagga taagatggac accttcatca agctgatcaa ggatgagatc atgaacaacg
     3241 gggccgtgat cgcctacgtg aaggccgaga atgtgctggg ctatgagctg aacggcaaga
     3301 acgtgcagaa cctgtgcggc gacaagaccc ccgaccacgc cgtgaatatc gtgggctacg
     3361 gcaactacat caacgacgag gatgagaaaa agtcctactg gatcgtgcgg aacagctggg
     3421 gcaagtactg gggcgacgag ggctacttca aagtggatat gtacggcccc tctacctgcg
     3481 aggacaattt catccacacc gtcgtggtgt ttaacatcaa tatgcccaag tccaagaagt
     3541 cccccgtcaa gattaccttc cccctgtaca actactacct gaagtacagc cccgacttct
     3601 accacaacct gtactacaag aatttcaaca gcaagaaatc catgaagctg gtcaacgccg
     3661 ccgacgagca caaaaacatc tatagccagg aggacaaagt caaccacaag aagggcagca
     3721 agattctgaa cagcgaagtg accaccagcc tgctgagcca ggaaatcagc cagcggagag
     3781 gcgaggatga cgatatcgat accctgatcg gcgactcccc cgatatcaac gagcaggaaa
     3841 aaaacattaa ggacgagttt aacaaggcca gcatcaccca caactccgtg tccagcagca
     3901 atatcgctaa gtccaacaag aataccaaca aagtgaagat ctatcacatc attaagcacg
     3961 tgaagaacac aaagatcaag atcggcttcg tgaagtatga caactacaac accatcggca
     4021 ccaaccacgc ctgcagcaga agctacagcg aggatcagga aaagcacgag ggctgcatca
     4081 agttttgcga gctgcactgg aacgagtgca aggacaagac cagccccggc tactgcctga
     4141 ccaagctgaa gggctccaat gagtgcttct tctgctacgt gggcgcgccg tcgacctcca
     4201 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     4261 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     4321 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     4381 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     4441 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     4501 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     4561 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     4621 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     4681 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     4741 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     4801 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     4861 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     4921 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     4981 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     5041 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     5101 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     5161 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     5221 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     5281 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     5341 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     5401 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     5461 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     5521 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     5581 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     5641 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     5701 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     5761 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     5821 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     5881 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     5941 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     6001 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     6061 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     6121 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     6181 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     6241 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     6301 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     6361 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     6421 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     6481 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     6541 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     6601 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6661 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     6721 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6781 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     6841 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     6901 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     6961 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     7021 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     7081 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     7141 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     7201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7621 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7921 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9241 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9301 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9361 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9421 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     9481 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     9541 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     9601 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     9661 tatgaccgc
//
