LOCUS       sequence-76306-e        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA6-bio-His
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4872..4889
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4941..4996
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4995..5014)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7232..7250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7318..7422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7423..7491,7492..8283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7641..7660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8454..9042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8943..8962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9196..9213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9330..9351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9366..9371,9372..9389,9390..9396)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9404..9420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9409..9431
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9428..9444
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9428..9444
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 acaaagtgac cgtgatcagc cacaacaacg gccacaacga caacctggac gtgaacaaga
     1261 acggcgtgat ctcccaggaa aatgtctttg acaccagcga gagcctgaac ctgcccagca
     1321 acaagaaagt gggcagcgac gacctgaaca ccgccaccat cagcttcacc gtgcccgaca
     1381 atctggaaaa cgaagtgaag gtggtgtcca gcagcgagtc cggaaagggc gccaccgtgt
     1441 cccacaccaa agtgacaagc gagggcctga gcgacaccca gcctaatgtg gcccagagcg
     1501 tgtccagctc cacccacaca cctggcagcc tggacagcac catgagcacc gagcagcaca
     1561 gcagcgtgtc ccagagcagc ctgcctacag agagcagcag cgaaaccctg aacaaggcca
     1621 ccgtgcctga gatccccatc cagatcaaca gcggcctgct gaagaactac aatggcgtga
     1681 aagtgacagg cagctgcggc agctacttcc gggtgtacct ggtgccccac atcctgatct
     1741 acgccctgac caagtactcc gtgattcagc tggaaagcct gttcaacgac aatgcccgga
     1801 tcgacgtgga acacaagggc gagctgcaga acaagtgcag cgagggctac cacttcaagc
     1861 tggtggtgta catcacccac aacgtgctga acctgaagtg gaaaacctac aagcccaacg
     1921 aggaaagcaa gagcgaggac agcgacgtgc ggaagtaccg gatccccaag ctggaacggc
     1981 ccttcaccag catccaggtg tacacagcca acagcaaggc tggcgtgatc gagacaaaga
     2041 attacaacat ccggaccgac atccccgata cctgcgacgc tatcgccacc gactgctttc
     2101 tgaacggcaa cgtgaacatc gagaagtgct tccagtgcac cctgctggtg cagaagaagg
     2161 acaagagcca cgagtgcttc aaatacgtgt cctccgagat gaagaaaaag atgaacgaga
     2221 tcaaagtgaa ggcccaggac gacttcaacc ccaacgagta caagctgatc gagagcatcg
     2281 acaacatcct gagcaagatc tacaagaagg ccaacaagcc cttcgagatc agcaaggacc
     2341 tgatcaacct ggaagatctg gactaccagt tcaagaacga gctgctcgag tactgcaagc
     2401 tgctgaaaaa ggtggacacc tccggcaccc tggaagagta cgagctgggc aacgccgagg
     2461 acatctacaa caacctggcc cggctgctga agtcccacag cgacgagaac atcgtgacac
     2521 tgcagggcaa gctgcggaat accgccatct gcatcaagaa cgtggacgag tggattctga
     2581 acaagagagg cctgacactg ccctccgaga gccctagcga gagcagctcc aagagcgaca
     2641 gctacctgaa taccttcaac gataaggata agaacgagga caaggacgac atgagcaaga
     2701 acagcaaaga ggaattcaaa aacgacgaca aagagaactc cgacgatcag aacaacaacg
     2761 acgccaacaa gaaggacgac gaaaacaaca tcaacaacgg ggacaccaac tacgtgtacg
     2821 acttcgacga cgacgattac gacaacaacg cctatgagaa ggatatgtac gagagcccca
     2881 tcaaagaaaa caagaatggg gtcatcgacc tcgagaagta cggcaaccag atcaagctga
     2941 agtctcccta cttcaagaac tccaagtact gtaactacga gtattgcaac cggtggcggg
     3001 acaagaccag ctgcatcagc cagatcgagg tggaagaaca gggcaattgc ggcctgtgct
     3061 ggatcttcgc cagcaagctg cacttcgaga caatccggtg catgcggggc tacggccact
     3121 tcagatccag cgccctgtac gtggccaact gcgccaagcg gaagcccatc gacagatgcg
     3181 aggaaggcag caaccccctg gaattcctgc ggatcctgga cgagaagaag ttcctgcccc
     3241 tggaaagcaa ctacccctac agctacacca gcgccggcaa cagctgccct aagctgccca
     3301 acagctggac caacctgtgg ggcgacacaa agctgctgtt caacaaaaag gtgcaccggt
     3361 acatcggcaa caagggcttc atcagccacg agacaagcta ctttaagaac aacatggacc
     3421 tcttcatcga catggtcaag cgggaagtcc agaacaaggg aagcgtgatc atctatatca
     3481 agacccagga cgtgatcggc tacgatttca acggcaaggg cgtgcacagc atgtgcggcg
     3541 acagaacccc tgaccacgcc gccaacatca tcggctatgg caactacatt aacaaaaaag
     3601 gcgagaagcg gagctactgg ctgatccgga acagctggtc ctactactgg ggcgacgagg
     3661 gcaacttcag agtggacatg ctgggcccca agaactgcct gtacaacttc atccacaccg
     3721 tggtgttctt caaactggac ctgggcacca tccacgtgcc caagaagaag tcttggaaga
     3781 agaacgtcta cttcctgcgg cacaaccccg acttcatgta cagcctgtac tacaacaatt
     3841 acgagcccga aaccagccag gacttcgaga gcgagaacga ctacgataac gccttcgtgc
     3901 acggccagag caacgaggcc gacgagacaa acaaagaggg caagaacgtc cacaacagcg
     3961 tggaaaagaa gattcagatc ctgcacatcc tcaagcacat caaggacagc cagattaagc
     4021 ggggcctcgt gaagtatgac aacattaacg aggccaaaga cgagcacacc tgtagcagag
     4081 tgaacagcca ggatgccgag aagtatgagg aatgcaagaa gttctgtctc accaagtgga
     4141 acgagtgcaa ggaccactac agccccggct actgcctgac cgacctgtat aagggcgagg
     4201 actgcaactt ctgctacgtg ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     4261 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     4321 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     4381 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     4441 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     4501 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     4561 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     4621 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     4681 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     4741 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     4801 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     4861 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     4921 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4981 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     5041 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     5101 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     5161 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     5221 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     5281 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     5341 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     5401 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     5461 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     5521 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     5581 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     5641 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     5701 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     5761 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     5821 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     5881 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     5941 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     6001 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     6061 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     6121 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     6181 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     6241 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     6301 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     6361 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     6421 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     6481 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     6541 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     6601 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     6661 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6721 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     6781 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     6841 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     6901 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     6961 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     7021 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     7081 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7141 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     7201 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     7981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8101 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9241 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9301 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9361 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9421 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     9481 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     9541 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     9601 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     9661 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
