LOCUS       sequence-76307-e        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA7-bio-His
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4623..4640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4692..4747
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4746..4765)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6983..7001)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7069..7173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7174..7242,7243..8034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7392..7411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8205..8793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8694..8713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8947..8964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9081..9102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9117..9122,9123..9140,9141..9147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9155..9171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9160..9182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9179..9195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9179..9195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcac
     1201 aggaaaagcc tccacctgat tctaccgtgg gcgccaaccc tggcgacgag agagaaagca
     1261 gcggcagagt gaacaacccc gcctctggcg agcagggcac caccaattct ccaaccgagc
     1321 agcccgacca gacccgggat agaagcagct ctgtgcctca gggcagcccc agagaacctg
     1381 tgtcccccga gaaccccaac cccgtgacac agatccctgg caatggcgga gccctggtca
     1441 cccctatccc tctgcctaag ctgaccctgg aagatagcga gagcagcaag agcgtgatcg
     1501 acatcgaagt gaagtccgcc ctgctgaaga actacgacgg cgtgaagatc accggcccct
     1561 gccggtccta cttcagagtg atgctggtgc cccacatcac cgtgtacgtg tacgccacct
     1621 acgaccggat tcagctggaa cccaagttcg gccccagcga cctgatcgat atcaacgacc
     1681 tgaccaacaa gtgcaacaag gacagcaaca agtacttcaa gctggtgctg tacatcaaga
     1741 acaacatcct gatcctgaag tggaaggtgc aggacaagga ctccaagccc accaacatcg
     1801 acgtggacgt gaagaagtac aagatcccca agctggaccg gcccttcacc tccatccagg
     1861 tgtacacagt gaacaccgag cacggcctga tcgagagcaa gaattacgac atcaacagcg
     1921 agatccccga gcagtgcgag gctatcagca ccaactgctt tctgaacggc gccctggacg
     1981 tggaaaactg ctaccactgt accctgctgg ccaagaaggt ggactccaac aacgagtgct
     2041 tcaactacgt gtccaaagag gccaaagagc tgatcaacaa gaacctggaa gagaagaaca
     2101 aggccttcaa gggcgaggac gaggacctgg acagcaatga gcagaagctg gaagaatcca
     2161 tcgacaatat cctgagcaac atctacaaga tctacgagtc caagcaggac aaagaacgga
     2221 agaagtccca ctacaacaac aagaaagagc tggtcaccat cgaggaactg aacagcgtgc
     2281 tgaagatcga gctgctgaac tactgcaagc tgctcaaaga ggtggaccgc agcggcatgc
     2341 tggaccacca cgagatcggc aacgagatcg acattttcaa caacctcatc cggctgctga
     2401 aggcccaccc aggcgagagc acctacgtgc tgaacgagaa gctgcggaac cccgccctgt
     2461 gcttcaagaa catcgaagag tggctcgtga acaagaaggg cctgctgctg agcaacgaga
     2521 agatccagaa cctggccacc accaactaca acgtggccga tctggaagag tccgagtacg
     2581 actacgagcg gttcatcagc gacgacatgt tcgagaagga catgaacggc gtcatcgacc
     2641 tgagcctgtt cgacaacgag aaaaagctga agtcccccta cttccggcgg aacaagtatt
     2701 gcaacaatga gtactgcgac cggtggaagg acaagaccgg ctgcatcagc aagatcgagg
     2761 tggaagaaca gggcaactgc ggcctgtgct ggatctttgc cagcaagctg cacttcgaga
     2821 caatccggtg catgcggggc tacggccact tcagatcttc cgccctgtat gtggccaact
     2881 gcgccgacag agacagcgac gagatctgct tcgtgggcag caatcccgtg gaattcctgg
     2941 aaatcgtgga agaaaccggc ttcctgcccc tggaaagcga cgtgccctac tactacaccg
     3001 acgccggcaa cgattgcccc gagcccgaga agaactggat caacctgtgg ggcagcacag
     3061 aactgctgaa ccacaagcgg cccagacagc ggatgaccac caagggctac atcagctacg
     3121 agtctagcta cttctccgac aacatggacc tgttcatcaa gatcatcaag cgggaaatcc
     3181 agaacaaggg ctccgtgatc gcctatatca agaccgagaa cgtgatcgat ttcgacttca
     3241 acggcaaggg cgtgcacaac atgtgcggcg acaaagagcc agaccacgcc gccaacatca
     3301 tcggctacgg caactacatc gacgaggaag gcgagaagaa gtcttactgg ctgatccgga
     3361 acagctgggg ctactactgg ggcgacgagg gcaatttcag agtggatatg tacggcccct
     3421 cttactgcaa gtacaacttc atccacaccg tggtggtgtt caaggtggac ctgggcatca
     3481 tcgaggtgcc aaagaaagag aaagagagcg agtacttcag ctacttcctg aagtacaccc
     3541 ccaactttct gtacaacctc ttcttcaaca actacgccac caatgacgag tacaagctga
     3601 acaaccggct caagaccaac cagcataaca acaaaaaaaa caaaaaggac cggtacatca
     3661 gcgcccagga cgagcccccc accgacaatg tggaaagcca ggccgagaac aacaaaaaga
     3721 ccgagatcta ccacatcctg aagcacatca aggataagaa gattaagcgg ggcctcgtga
     3781 agtacgagag cctgctggaa actaagaagg accacagctg cagccggacc cacagcatcg
     3841 accccgagaa gcacgaggaa tgcaaccagt tctgcatcga caactggaag gcctgcaagg
     3901 accactacag ccccggctac tgcctgacca agctgtacac caaggacgac aactgcttct
     3961 tctgcaacgt gggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     4021 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     4081 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     4141 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     4201 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     4261 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     4321 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     4381 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     4441 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     4501 ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac atcctggacg
     4561 cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc cctctgggcc
     4621 cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc ccccgacctc
     4681 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     4741 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     4801 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     4861 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     4921 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     4981 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     5041 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     5101 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     5161 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     5221 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     5281 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     5341 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     5401 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     5461 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     5521 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     5581 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     5641 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     5701 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     5761 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     5821 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     5881 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     5941 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     6001 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     6061 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     6121 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     6181 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     6241 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     6301 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     6361 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     6421 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6481 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6541 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     6601 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     6661 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     6721 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     6781 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     6841 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     6901 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     6961 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7021 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7081 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7141 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7201 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7261 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7321 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7381 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     7441 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7501 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7561 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7621 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7681 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     7741 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7801 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7861 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7921 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8041 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8101 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8161 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8221 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8281 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8341 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8401 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8461 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8521 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8581 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8641 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8701 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8761 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8821 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8881 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8941 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9001 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9061 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9181 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     9241 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     9301 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
     9361 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     9421 atatgtacat ttatattggc tcatgtccaa tatgaccgc
//
