LOCUS       sequence-76326-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-Rab28 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  TITLE     Scidmore Lab GFP-Rab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFP-Rab28
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             120..128
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    858..979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(895..914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     949..968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc caccatgtcg gattcggagg
       61 aggagagcca ggaccggcaa ctgaaaatcg tcgtgctggg ggacggcgcc tccgggaaga
      121 cctccttaac tacgtgtttt gctcaagaaa cttttgggaa acagtacaaa caaactatag
      181 gactggattt ctttttgaga aggataacat tgccaggaaa cttgaatgtt acccttcaaa
      241 tttgggatat aggagggcag acaataggag gcaaaatgtt ggataaatat atctatggag
      301 cacagggagt cctcttggta tatgatatta caaattatca aagctttgag aatttagaag
      361 attggtatac tgtggtgaag aaagtgagcg aggagtcaga aactcagcca ctggttgcct
      421 tggtaggcaa taaaattgat ttggagcata tgcgaacaat aaaacctgaa aaacacttac
      481 ggttttgcca ggaaaatggt tttagtagcc actttgtctc agccaagaca ggagactctg
      541 tcttcctgtg ctttcagaaa gttgctgctg aaatccttgg gatcaaatta aacaaagcag
      601 aaatagaaca gtcacagcgt attgtcaggg cagaaatagt gaagtacccg gaagaagaaa
      661 atcaacatac cacctctact cagagtagaa tctgttcagt acagtagctc gacggtaccg
      721 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
      781 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      841 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      961 aactcatcaa tgtatcttaa cgcgtaaat
//
