LOCUS       sequence-76408-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45125
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(748..766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
       61 actctgtggt ctcaggagat atgactggta agtttttctt gccaatacga attagaaaac
      121 atgtctttga agatgaactg tatttttttt ttttactttg ttgtcatttt aatgtacttt
      181 cttatcagga ttaaatcttc tgtaatttag agtagttttt ttaacaagat aattaacaaa
      241 cttagagtaa tgaaaattga gatgttcagt tttcactcat atttcacatt ttggtgaaag
      301 agtgggtagt atgcaacgtt ctaagtatgt ttggactttg tatcatgttg ttttgattct
      361 ttgacgacat gtctatttgg gaaacaccaa tgacgtgtac cttgagactg atacgattca
      421 aagggataga aacacgtcag atttacaagt ggcacctctt caatggacaa tgggtattcc
      481 aatatgctaa gatgctacga gatatctaat ttatctaaca caactcaatt ccaaaccaaa
      541 aatctgatgc cagctcgaca agacaaaaaa tctaagctca aaaatgtcaa caaccaatag
      601 aaatcaaggc attgacgata tcacgagata agcaaattaa atcttcaagt tttgcaattc
      661 atatgtacgt tataaatacc caaaaacctc accgtaacct agctatccta cttgagacca
      721 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      781 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aga
//
