LOCUS       sequence-76422-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH77901
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      289..541
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(581..599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        667..771
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagct tggactccca
      301 tgttggcaaa ggcaaccaaa caaacaatga atgatccgct cctgcatatg gggcggtttg
      361 agtatttcaa ctgccatttg ggctgaattg tagacatgct cctgtcagaa attccgtgat
      421 cttactcaat attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt
      481 tgtattgttt catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat
      541 ccgcttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
      601 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      661 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
      781 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
      841 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tcc
//
