LOCUS       sequence-76432-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50010
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             290..379
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             320..409
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(449..467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        535..639
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactgtgg tctcattcgg aacaaaagtt
      301 gatctctgaa gaggaccttg agcagaaatt gatctctgag gaagatcttg agcagaagct
      361 tatctcagag gaggatttgg agcaaaaact tatttctgaa gaggaccttt gagctttgag
      421 accacgtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
      481 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
      661 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
      721 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
      781 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
      841 ctaaaacaaa gttaaacatc atga
//
