LOCUS       sequence-76525-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30010
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(799..817)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatca ctcgtggtct cacattctcc tagcgttgtc aggcaatcca gagtgtgcca
      121 ctgcatgctc gtacaactta accttattgt agtccttgtc gtgagacaaa atctcgatgc
      181 agtggtcaac gaagtggtat ccaggcaact tcactccctt ctccttagcc ttgtatgtag
      241 tcctgaagtc gcacctgtag tgagcgttac cctcaagcaa caatgccatg tggatgtcac
      301 cagtcaacac tccgtccctc acgtacatct tctctgtaga aggctcccat ttcaatgttt
      361 ttttctgcat cacaggtccg tttgcaggga agttagttcc gtagaacctc accttgttgt
      421 aaaatgtgtc tccctccatt gtgatgtcgt tccttgcgat gcagatacct ccgtcctcga
      481 aagtcaatga tctctcccaa gagtaaccct ttgggaatga ctgcttgaag taatcttgaa
      541 tgttatcagg gtacttagcg aaaaccctgt taccgtagtg gaaagcagta gtcaagatgt
      601 cgaaagcgaa tggcaaaggt cctccctcct tcacctccaa gtccattgac tgctttccct
      661 cgaaaggctt accagttccg tcaccgtcga tcacgaagtg gtgtccgttc acgtttccct
      721 ccatcctcaa cttgatcttc atgtcaggct taattgcaga catggtgaga ccacagtggc
      781 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      841 taatggtttc tta
//
