LOCUS       sequence-76547-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQF-lacZ
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattcaat acgggcagac atggcctgcc
      241 cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg cgctcagctg
      301 taattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca tatggaaacc
      361 gtcgatattc agccatgtgc cttcttccgc gtgcagcaga tggcgatggc tggtttccat
      421 cagttgctgt tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg
      481 gccataattc aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga agacgtacgg
      541 ggtatacatg tctgacaatg gcagatccca gcggtcaaaa caggcggcag taaggcggtc
      601 gggatagttt tcttgcggcc ctaatccgag ccagtttacc cgctctgcta cctgcgccag
      661 ctggcagttc aggccaatcc gcgccggatg cggtgtatcg ctcgccactt caacatcaac
      721 ggtaatcgcc atttgaccac taccatcaat ccggtaggtt ttccggctga taaataaggt
      781 tttcccctga tgctgccacg cgtgagcggt cgtaatcagc accgcatcag caagtgtatc
      841 tgccgtgcac tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc
      901 gacccaggcg ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg
      961 tgcacgggtg aactgatcgc gcagcggcgt cagcag
//
