LOCUS       sequence-76548-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL13002
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        385..730
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     673..693
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(773..791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc
      301 tcaggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
      361 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
      421 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      481 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
      541 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
      601 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      661 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      721 ggagaggaca cgcccattga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
      781 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      901 atgtatccgc tcatgagaca ataaccctga taaatgcttc a
//
