LOCUS       sequence-76566-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc31_34
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             345..356
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgccaaaaca gggatcctta accacgtttc acttcacatt cacccagatg acgttttttt
       61 tctgctgcaa tcagaatcgg cagaactgcc agaacacaaa cctgcagaac aataaaaact
      121 gccggtgcct gggtaaatgc cagaattgct gcgctaacaa ccatcagggc acaaaacaca
      181 aacagaatga tttcttgcag cagtttttta cgatattctg ctttcatctg gctcagctga
      241 tgcagcagtt ctttctgatt cggaatgctc ggatttgcgg tctgatcgat ttttttccac
      301 tcgcttttca ggtggtcgct cagtttttct ttgttcatat ggtatttctc ctctttctct
      361 agtatttatt cgactataac aaaccatttt cttgcgtaaa cctgtacgat cctacaggtc
      421 agccaacgat cgttccgatc agaacaagaa agctatgtgg cctgacgaag cggcgcgcca
      481 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
      541 gggtggtgaa tatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta
      601 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac
      661 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt
      721 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa
      781 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat
      841 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag
      901 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta
      961 gttttgcaca ttcagaaaaa gacaactata tagatag
//
