LOCUS       sequence-76641-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30004
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     337..355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(436..457)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(697..716)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
       61 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
      181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      361 cgtccactga agagccactg tggtctcacc atggtgagca agggcgagga gctgttcacc
      421 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      481 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      541 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
      601 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      661 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      721 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      781 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactac
//
