LOCUS       sequence-76646-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70004
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    76..130
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(186..205)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     796..815
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
       61 tctcctattt ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat
      121 ttacaattac catggttgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      181 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      241 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      301 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      361 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      421 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      481 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      541 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      601 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
      661 gcatgcccga cggcgaggat ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca
      721 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
      781 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
      841 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
      901 atcgccttct tgacga
//
