LOCUS       sequence-76726-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM1479
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(169..187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        255..359
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgtggtctc atactgtata agagctcatt tttacaacaa ttaccaacaa caacaaacaa
       61 caaacaacat tacaattaca tttacaatta tcgatacatg ggcagccacc accaccacca
      121 ccacagcgga atgtgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
      181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      361 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
      421 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
      481 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
      541 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
      601 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
      661 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
      721 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
      781 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgta
//
