LOCUS       sequence-76767-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with 3 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-3XDSR
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..32
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(47..52,53..70,71..77)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    85..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     90..112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     109..125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     109..125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(144..177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
       61 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      121 atgaccatga ttacgccaag cttataactt cgtatagcat acattatacg aagttattgc
      181 tagccgtttt cttcaattac taaattagaa ctaattcaat catatgctgg catgcggaga
      241 gctcggagtc gacgagacta gtactggttc tacaggatca accggctcca cggggtcgat
      301 gggtagatct atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
      361 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
      421 tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg
      481 gccaacctta gtcactactt taacttatgg tgttcaatgt ttttctagat acccagatca
      541 catgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
      601 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
      661 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt
      721 aggtcacaaa ttggaataca actat
//
