LOCUS       sequence-76779-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with 8 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-8XDSR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..850)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(137..850)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 acttcgtata taataccata tacgaagtta tcccggggtt taacataaag tttaagcacc
       61 tgtttaacat tacgtttaac ctgccgttta aagttctgtt taagaatggg tttaatacgg
      121 agtttaaaga tctttatttg tacaattcat ccataccatg ggtaatacca gcagcagtaa
      181 caaattctaa caagaccatg tggtctctct tttcgtttgg atctttggat aaggcagatt
      241 gagtggataa gtaatggttg tctggtaaca agactggacc atcaccaatt ggagtatttt
      301 gttgataatg gtcagctaat tgaacagaac catcttcaat gttgtgtcta attttgaagt
      361 taactttgat accattcttt tgtttgtcag ccatgatgta aacattgtga gagttatagt
      421 tgtattccaa tttgtgacct aaaatgttac catcttcttt aaaatcaata ccttttaatt
      481 cgattctatt aactaaggta tcaccttcaa acttgacttc agctctggtc ttgtagttac
      541 cgtcatcttt gaaaaaaata gttctttctt gaacataacc ttctggcatg gcagacttga
      601 aaaagtcatg ttgtttcatg tgatctgggt atctagaaaa acattgaaca ccataagtta
      661 aagtagtgac taaggttggc catggaactg gcaatttacc agtagtacaa ataaatttta
      721 aggtcaattt accgtaagta gcatcacctt caccttcacc ggagacagaa aatttgtgac
      781 cattaacatc accatctaat tcaaccaaaa ttgggacaac accagtgaat aattcttcac
      841 ctttagacat agatctaccc atcgaccccg tgg
//
