LOCUS       sequence-76791-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN501
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(322..342)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(349..414)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(597..941)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(633..653)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cctgtggaag aaagaatcgt caaccttcgc catctcgttg gagaaaatct cctgcaggta
       61 gcagatgcga ttcttcctgc gcgtgtaccg cctgcgggcg gtgcgcttga gccgcgtagc
      121 ctcagccgtc tcgccgctgt cgaacaggag agcgccaatg agattcttct tgatggaatg
      181 ccgatcggtg ttgcccagga ccttgaactt ctttgagggc accttgtact cgtcggtgat
      241 cacggcccag ccaacagagt tagtcccaat ggcgaggccg atcgagtact tcttgtcagc
      301 agctggcacc ccgtggatgc caaccttcct cttcttcttc ggagccatct tgtcatcatc
      361 atccttgtaa tcaatgtcgt ggtccttgta atccccgtcg tggtccttgt aatccatcta
      421 gagcggccgc ggccatacaa gtattgggga tccccctcga gtatcgttcg taaatggtga
      481 aaattttcag aaaattgctt ttgctttaaa agaaatgatt taaattgctg caatagaagt
      541 agaatgcttg attgcttgag attcgtttgt tttgtatatg ttgtgttgag gtcgaggtcc
      601 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
      661 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
      721 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
      781 tgtcggcaga ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg
      841 agccaccttc cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc
      901 cgaggaggtt tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg
      961 agactgtatc tttgatattt tt
//
