LOCUS       sequence-76795-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-DT1T2
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(502..577)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(644..663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(645..663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(670..687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(670..686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(679..701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaactgccat tgtataactt tgccaatctc ttagtcgact ctaccaatat ataaacagag
       61 cttctatttt caactgaacg atgtgggatg tttttgacat cgtttacagc ttaaacgggc
      121 tcgtttatta aaagccctct tctttcgatc catcaacatt attggcctta agtaaaacaa
      181 gcctctttat gattgagaaa acgccgtcgt tggtgcacca gagagaagaa acaaaacagc
      241 tagcgttaga taaactttaa aattgtttgc atcctcattt gtctgtcagg ctgcagtagt
      301 ttggattaat attggtttat ctcatcggaa ctgcaaaact caactaactg aaaagtacaa
      361 aaccactgaa tcatattatt tgagattttt tttaggtcaa attttaggtt tcagttacag
      421 aaaacgaaga gaaaacccca gaaattgaac gccgaagaac agaggaagaa gaaatcgatc
      481 tggaaaattt tgcaaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      541 tagccttatt ttaacttgct atttctagct ctaaaacgag aaccgccatg gccgtccaat
      601 agagaccatt attcctttgg cctatttggc ctggcccaat tcgccctata gtgagtcgta
      661 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      721 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      781 caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac
      841 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
      901 gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtca
//
