LOCUS       sequence-76986-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the endogenous promoter in the pRS414 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Abeliovich H, Zhang C, Dunn WA Jr, Shokat KM, Klionsky DJ
  TITLE     Chemical genetic analysis of Apg1 reveals a non-kinase role in the 
            induction of autophagy.
  JOURNAL   Mol Biol Cell. 2003 Feb;14(2):477-90.
  PUBMED    12589048
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2003-02"; volume: "14(2)"; pages: "477-90"
COMMENT     Sequence Label: GFP-ATG8(414)/GFP-AUT7(414)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     33..49
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..668)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat
       61 atcgaattcg aaatcttggc tccgttgtgt acaaaacttc ttaatgaata tatatatatt
      121 tttcccttat tttatctttt tttttcgaat tttttatgta aacattctta tactggaaca
      181 atagatggct aatgagtccc tataatttcg attttagatg ttaacgcttc atttcttttc
      241 atataaaaga ctacctgcca aatgtatttt ctcctgagta agtgacatac aaaaacccgt
      301 ccttatcctt gtgttcttga tatatggcag acatcaacgc cgcagtaggt ggcaaagtat
      361 cattgacaaa aatgaagatg gccttctcag ggggtagcat aattctcttt cttataacat
      421 aaacaaattg ccctacggta aggtcagcag gaactagata tttacgctta tcaatctctg
      481 gaatatctga cttttcagct ttttcgcaaa tcacaggtat cctattcttg aacctgtcag
      541 caatcctctc cgactccgcc ttcctttttt caaatggata ttcagactta aatgtagact
      601 tggatccttt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      661 gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca
      721 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      781 ggtctgctag ttg
//
