LOCUS       sequence-76990-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pcDNA3.1-nAChr-Alpha6-mCherry
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(291..998)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(566..585)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     835..853
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgggccctc tagactcgag ttaagacttt cctgtgttcc caagcagtgg ctgcagaaat
       61 agccccacag ttccaaacac acagacaatt ataaataccc aaaggaagac tctgtccacc
      121 accatagcca tgtatttcca gtcgtcttct acctcgtttg tttcattgtg gctcttcatg
      181 ttttctgcta tgaattgaac actctcgatg acatcttcaa catcggacga gtgctctgaa
      241 ttctctgcca cccaccgtgc aggctgctgg cttgatcttc tctttccagg cttgtacagc
      301 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
      361 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
      421 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
      481 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      541 tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
      601 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
      661 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
      721 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
      781 ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc
      841 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
      901 tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg
      961 atgatggcca tgttatcctc ctcgcccttg ctcaccattg ctatgtcact tgatttttgg
     1021 cagtggtggc attcctttag aa
//
