LOCUS       sequence-77008-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the endogenous promoter in the pRS414 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Abeliovich H, Zhang C, Dunn WA Jr, Shokat KM, Klionsky DJ
  TITLE     Chemical genetic analysis of Apg1 reveals a non-kinase role in the 
            induction of autophagy.
  JOURNAL   Mol Biol Cell. 2003 Feb;14(2):477-90.
  PUBMED    12589048
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2003-02"; volume: "14(2)"; pages: "477-90"
COMMENT     Sequence Label: GFP-ATG8(414)/GFP-AUT7(414)
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..32
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(693..721)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacgccagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
       61 gcggccgctc tagaactagt aggtctcgca agagagctgg tcaacagaat ccaaaaattg
      121 agaaagaagt gtggtttgga agccaccgac gatgttttag tggagtacga attagttaaa
      181 gatactatcg actttgaagc cattgtcaaa gaacattttg atatgttaag caagacctgt
      241 agatccgaca ttgccaaata tgacggctca aagacagacc caattggtga tgaagaacaa
      301 tctattaatg acaccatttt caaattaaaa gtgttcaaat tatgaaaaca actcatataa
      361 atacgtacaa atttttctct actcgaagtg atatagatgt atatgtgtaa gtttacgttt
      421 aagttagagt catgtaatgc taactgtctc caccgataat gttgtataat acccgtgaaa
      481 tcatagcaca tgatatatca tcacccggag gccggttatt ttcggcggcg gcaaaaatat
      541 ttggtataat tatggaaata caaaaagggg aaccattaaa ggttgaggag gggattgata
      601 agagaatcta ataattgtaa agttgagaaa atcataataa aataattact agagacatgg
      661 gatccggtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      721 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      781 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      841 ttgtcactac tttcacttat ggtgttcaat gcttttca
//
