LOCUS       sequence-77019-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a CMV promoter when no GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle-CMV
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   310..412
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 tgagcgtcga tttttgtgat gctccgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
      241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg atccatgcat
      301 gttaattaac atcatcaata atatacctta ttttggattg aagccaatat gataatgagg
      361 gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt aggttttagg
      421 gcggagtaac ttgtatgtgt tgggaattgt agttttctta aaatgggaag tgacgtaacg
      481 tgggaaaacg gaagtgacga tttgaggaag ttgtgggttt tttggctttc gtttctgggc
      541 gtaggttcgc gtgcggtttt ctgggtgttt tttgtggact ttaaccgtta cgtcattttt
      601 tagtcctata tatactcgct ctgcacttgg ccctttttta cactgtgact gattgagctg
      661 gtgccgtgtc gagtggtgtt tttttaatag gttttctttt ttactggtaa ggctgactgt
      721 tatggctgcc gctgtggaag cgctgtatgt tgttctggag cgggagggtg ctattttgc
//
