LOCUS       sequence-77020-e         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo GFP based system to measure break induced replication (BIR) 
            repair efficiency. expression of I-SceI generates a double strand 
            break that when repaired by BIR mechanism will restore GFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  TITLE     pBIR-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBIR-GFP
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..219
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     281..300
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             358..378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             400..420
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
       61 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
      121 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
      181 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      241 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      301 ctgtctcatc attttggcaa agaattcaga tccgccgcca ctatgggatc aagatcgcca
      361 aaaaagaaga gaaaggtgcc gaagaagcat gcagcaccac caaaaaaaaa acgaaaagta
      421 gaagacccac gaggcaacac cagcggcgtg ctgagcaccc ccaaggccaa gagggccaag
      481 cacccccccg gcaccgagaa gcccaggagc aggagcca
//
