LOCUS       sequence-77021-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50571.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-M
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..267)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     233..251
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(277..504)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(532..558)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gggcgattgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca
       61 aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
      121 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
      181 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
      241 gtaagcgtga cataactaat tacatgactc gagttaacca cctcttagcc ttagcacaag
      301 atgtaaggtg gactccttct gaatgttgta atcagacagc gttctaccgt cttctagctg
      361 cctaccggca aagatcaatc tttgttgatc tggagggata ccttccttgt cttgaatttt
      421 cgacttaacg ttgtcgatgg tatcggaaga ttcaacttcc aatgttatgg ttttaccggt
      481 caaagtcttg acgaaaatct gcatggtcga cccactaccc gctaaaaatg cagcgtaatc
      541 tggaacatcg tatgggtagg taccgtcttt cttctcgtag acttcaaact tatacttgat
      601 gcctttttcc tcctggacct cagagaggac gcctgggtat tctgggagaa gtttatactt
      661 ccccaaatca atttctggga aaaacgtgtc actttcaaat tcctgcatga tccttgtcac
      721 aaagagtctg aggtggcctg gttgattcat ggcttcctgg taaacagaac tgcctccgac
      781 tatcc
//
