LOCUS       sequence-77022-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50571.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-M
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..472
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    31..148
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     443..466
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     487..503
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     487..503
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctaaagggaa caaaagctgg agctctagta cggattagaa gccgccgagc gggtgacagc
       61 cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg
      121 cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat
      181 ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc
      241 aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt
      301 aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa
      361 ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata
      421 aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc
      481 ccggattcta gaactagtgg atcccccggg ctgcaggaat tcatggttcg accattgaac
      541 tgcatcgtcg ccgtgtccca aaatatgggg attggcaaga acggagacct accctggcct
      601 ccgctcagga acgagttcaa gtacttccaa agaatgacca caacctcttc agtggaaggt
      661 aaacagaatc tggtgattat gggtaggaaa acctggttct ccattcctga gaagaatcga
      721 cctttaaagg acagaattaa tatagttctc agtagagaac tcaaagaacc accacgagga
      781 gctcattttc ttgccaaaag tttggatgat gccttaagac ttattgaaca accggaattg
      841 gcaagtaaag tagacatggt ttggatagtc
//
