LOCUS       sequence-77029-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with 
            start and stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..815)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(185..208)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(383..402)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     652..670
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(816..836)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(868..886)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(869..886)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 caaactcacc ctgaagttct caggatcggt cgagatcctt acttagttac ccgggaggat
       61 gagggtctca cttaggtacc ctacttgtca tcgtcatcct tgtaatcctt gtacagctcg
      121 tccatgccgc cggtggagtg gcggccctcg gcgcgctcgt actgttccac gatggtgtag
      181 tcctcgttgt gggaggtgat gtccagcttg atgttgacgt tgtaggcgcc gggcagctgc
      241 acgggcttct tggccttgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc
      301 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
      361 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
      421 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
      481 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      541 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
      601 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
      661 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
      721 aactcgcggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
      781 atggccatgt tgtcctcctc gcccttgctc accattacct tacgcttctt ctttggagcc
      841 atggcggcgg atccggtagt tgagacctct atagtgtcac ctaaatcaag cttgctccga
      901 cgtcccc
//
