LOCUS       sequence-77056-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express myc and SNAP-tagged GP135 in mammmalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pCDNA3.1(+)-myc-SNAP-GP135
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             170..199
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             203..748
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
       61 gagctcggat ccactagtcc agtgtggtgg aattcgcacg atgcgccccg cgccgccgcc
      121 gccgctgctg ctgctgctgc tgctgctgcc gccgccgtcg ctgtcgcacg agcagaagct
      181 gatctccgag gaggacctcg agatggacaa agactgcgaa atgaagcgca ccaccctgga
      241 tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt
      301 cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct
      361 gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact ttcaccagcc
      421 tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag
      481 ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag
      541 ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc
      601 cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacct
      661 ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg
      721 ccacagactg ggcaagcctg ggctgggtgg cggccgcctc gagatcgcag caacatctcc
      781 aacctcagg
//
