LOCUS       sequence-7707-ex       10094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, 
            Sheppard D
  TITLE     The cytoplasmic domain of the integrin alpha9 subunit requires the 
            adaptor protein paxillin to inhibit cell spreading but promotes cell
            migration in a paxillin-independent manner.
  JOURNAL   Mol Biol Cell. 2001 Oct . 12(10):3214-25.
  PUBMED    11598204
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2001 Oct . 12(10):3214-25."
COMMENT     Sequence Label: integrin alpha9 / alpha5 pcDNA1 neo
FEATURES             Location/Qualifiers
     source          1..10094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            join(1204..1278,1279..1363,1364..1383)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        1542..1921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1922..2125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5430..5448)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5431..5448)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          5532..5597
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5727..5747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6019..6153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6181..6200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6477..6739
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7099..7893
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7153..7172)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7763..7782
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8314..8379
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8509..8529
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8954..9088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9935..10070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     1261 agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     1321 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     1381 aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     1441 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     1561 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1621 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1681 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1741 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1801 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1861 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1921 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1981 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2041 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2101 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2161 tatcgaaatt aatacgactc actataggga gacccaagct tagagcgggc gctatgggga
     2221 gccggacgcc agagtcccct ctccatgccg tgcagctgcg ctggggcccc cggcgccgac
     2281 ccccgctact gccgctgcta ttgctgctcg tgccgccgcc accgcgggtc gggggctaca
     2341 acctggatcc gcagcgcccc gtgcacttcc agggccccgc tgactcgttc ttcggctacg
     2401 cagttctgga gcatttccac gacaacacgc gctgggtcct tgtgggcgca ccaaaggcag
     2461 attccaaata cagcccttca gtgaagtctc ctggggctgt gtttaagtgc cgtgttcaca
     2521 ccaaccctga ccggagatgc accgaactgg acatggctcg agggaagaat cggggcacgt
     2581 cctgcggaaa gacctgccgg gaagaccgcg atgatgagtg gatgggggtg agcctggccc
     2641 gacagcccaa ggctgatggc cgtgtgttgg cctgtgctca tcgctggaag aacatctact
     2701 atgaagccga ccacatccta ccccatggct tctgctacat catcccctcc aacctccagg
     2761 ccaaaggcag gacgctgatc ccttgctatg aagagtataa gaagaagtac ggagaggaac
     2821 acggctcctg ccaggctggg atagcgggct tcttcaccga ggagctggtg gtgatgggtg
     2881 ctccagggtc attttattgg gctggaacca tcaaagtgct gaaccttacg gacaacacct
     2941 atttaaaact gaacgacgaa gtgatcatga acaggcggta cacctacctg ggctacgcag
     3001 tgaccgctgg ccacttctct cacccgtcca ccattgatgt ggtaggaggt gccccacagg
     3061 acaaaggcat cggcaaggtt tatattttca gagctgaccg aagatcaggc accttaatta
     3121 agatctttca agcatcaggt aaaaagatgg gctcttactt cggctcctcc ttgtgcgcag
     3181 ttgacctgaa tggggacggc ctctctgacc tgctggtggg ggcccccatg ttttctgaga
     3241 tcagggatga gggacaggtc actgtctaca tcaacagagg aaatggagcc ctcgaggagc
     3301 agctggctct gactggggat ggtgcctaca atgcccactt tggagagagc attgccagcc
     3361 tggacgatct ggacaatgat gggttcccag atgtggccat tggtgcaccc aaggaggatg
     3421 acttcgcagg ggcggtctat atctatcatg gtgatgccgg tgggatagtc cctcagtact
     3481 caatgaaact gtctgggcag aagataaatc cagtgctccg gatgtttggt cagtccatat
     3541 cgggaggcat tgatatggat ggaaatggct atcctgatgt cactgttgga gccttcatgt
     3601 ccgacagcgt ggttcttctc agagcaaggc ctgtcattac ggtggatgtc tccatcttcc
     3661 tcccgggctc catcaacatc acagcgcctc agtgtcacga cggacagcag cctgtgaact
     3721 gcctgaacgt caccacctgc ttcagcttcc atggcaaaca cgttccagaa gagattggcc
     3781 tgaattatgt tctgatggct gacgtggcca aaaaggagaa gggccagatg cccagggtct
     3841 actttgtgct gctgggagag accatgggtc aggtcacaga gaagctgcag ctgacttaca
     3901 tggaggagac gtgtcgtcac tatgtggccc atgtgaagcg gagggtgcag gacgtcatca
     3961 gcccgatcgt gtttgaagca gcctacagcc tcagtgagca tgtgactgga gaggaggaga
     4021 gggaactgcc gcctctgaca ccagttctcc gctggaaaaa gggacaaaag attgcccaaa
     4081 agaatcagac tgtttttgaa aggaattgcc gttcagagga ctgtgccgca gacctgcagc
     4141 ttcagggtaa actgctgctc tccagtatgg atgagaaaac cctgtatcta gctttggggg
     4201 ctgtgaagaa catctcccta aacatctcta tctccaacct cggagatgat gcctatgatg
     4261 ccaacgtgtc cttcaatgtt tcccgggagc tcttcttcat caacatgtgg cagaaggagg
     4321 agatgggcat ctcctgtgag ctgctggaat cggacttcct caaatgcagc gtgggatttc
     4381 ctttcatgag gtcaaagtca aagtatgaat tcagcgtgat ctttgataca agccacctgt
     4441 ctggggaaga ggaagttctc agcttcattg ttactgctca gagtggcaac acggagcgct
     4501 ctgaatccct gcatgacaac accctcgtgc tgatggtgcc actgatgcac gaggtggaca
     4561 cgtccatcac cggaatcatg tctccaacct cctttgtata tggcgagtcc gtggacgcag
     4621 ccaacttcat tcagctggat gacctggagt gtcactttca gcccatcaat atcacccttc
     4681 aggtctacaa cactggccca agcacccttc cagggtcatc tgtcagcatc tctttcccta
     4741 atcgactctc atctggtggt gcagagatgt ttcatgtcca ggaaatggtg gtgggccaag
     4801 agaagggaaa ctgctctttc cagaaaaacc caactccctg catcatccct caagaacaag
     4861 aaaatatctt ccacacaata tttgcttttt tcacaaagtc tggaagaaaa gtcttggact
     4921 gtgaaaaacc aggaatttct tgcctaacag cacactgtaa ctttagtgct cttgctaaag
     4981 aagaaagtcg tactatagac atttacatgc tgctgaacac agaaatactg aaaaaggaca
     5041 gttcgtctgt catccagttc atgtcccgcg ccaaggtgaa ggtggatcct gccctaaggg
     5101 tggtggaaat agctcatggg aacccagaag aggtgacggt ggtcttcgag gccctgcaca
     5161 atctggagcc ccgtggctac gtcgtggggt ggatcatcgc catcagtttg ttggtgggaa
     5221 tcctcatctt cctgctgctg gccgtactag tctggaaact tggattcttc aaacgctccc
     5281 tcccatatgg caccgccatg gaaaaagctc agctcaagcc tccagccacc tctgatgcct
     5341 gagtcctccc aatttcagac tcccattcct gaagaaccag tccccccact ctagagcggc
     5401 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagaggatct
     5461 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     5521 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     5581 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     5641 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     5701 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     5761 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     5821 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     5881 aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     5941 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     6001 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6121 aaactcatca atgtatctta tcatgtctgg atctcccgat cccctatggt gcactctcag
     6181 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     6241 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     6301 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     6361 atatacgcgt atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta
     6421 cgcggttagg agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg
     6481 tagtcttatg caatacactt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     6541 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     6601 gccttattag gaaggcaaca gacaggtctg acatggattg gacgaaccac tgaattccgc
     6661 attgcagaga taattgtatt taagtgccta gctcgataca ataaacgcca tttgaccatt
     6721 caccacattg gtgtgcacct cctaagctag ctttgccgca agcactcagg gcgcaagggc
     6781 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     6841 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     6901 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     6961 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     7021 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     7081 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7141 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7201 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7261 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     7321 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7381 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7441 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7501 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7561 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7621 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7681 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7741 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7801 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7861 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     7921 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7981 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8041 tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct
     8101 gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg
     8161 aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga
     8221 tggccgcttt ggtcccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     8281 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     8341 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     8401 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     8461 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     8521 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     8581 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     8641 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     8701 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     8761 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     8821 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     8881 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     8941 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9061 catcaatgta tcttatcatg tctggatcga tcccgccatg gtatcaacgc catatttcta
     9121 tttacagtag ggacctcttc gttgtgtagg taccgctgta ttcctaggga aatagtagag
     9181 gcaccttgaa ctgtctgcat cagccatata gcccccgctg ttcgacttac aaacacaggc
     9241 acagtactga caaacccata cacctcctct gaaataccca tagttgctag ggctgtctcc
     9301 gaactcatta caccctccaa agtcagagct gtaatttcgc catcaagggc agcgagggct
     9361 tctccagata aaatagcttc tgccgagagt cccgtaaggg tagacacttc agctaatccc
     9421 tcgatgaggt ctactagaat agtcagtgcg gctcccattt tgaaaattca cttacttgat
     9481 cagcttcaga agatggcgga gggcctccaa cacagtaatt ttcctcccga ctcttaaaat
     9541 agaaaatgtc aagtcagtta agcaggaagt ggactaactg acgcagctgg ccgtgcgaca
     9601 tcctctttta attagttgct aggcaacgcc ctccagaggg cgtgtggttt tgcaagagga
     9661 agcaaaagcc tctccaccca ggcctagaat gtttccaccc aatcattact atgacaacag
     9721 ctgttttttt tagtattaag cagaggccgg ggacccctgg gcccgcttac tctggagaaa
     9781 aagaagagag gcattgtaga ggcttccaga ggcaacttgt caaaacagga ctgcttctat
     9841 ttctgtcaca ctgtctggcc ctgtcacaag gtccagcacc tccatacccc ctttaataag
     9901 cagtttggga acgggtgcgg gtcttactcc gcccatcccg cccctaactc cgcccagttc
     9961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10021 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    10081 caaaaagcta attc
//
