LOCUS       sequence-77072-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  purification of Dicer1 from mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Betancur JG, Tomari Y
  TITLE     Dicer is dispensable for asymmetric RISC loading in mammals.
  JOURNAL   RNA. 2012 Jan;18(1):24-30. doi: 10.1261/rna.029785.111. Epub 2011 
            Nov 21.
  PUBMED    22106413
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.029785.111"; journalName: "RNA"; date: "2012-01"; 
            volume: "18"; issue: "1"; pages: "24-30"
COMMENT     Sequence Label: pCAGEN-SBP-DICER1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..113
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    129..153
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggacgagaa gaccaccggc tggcggggcg gccacgtggt ggagggcctg gccggcgagc
       61 tggagcagct gcgggccagg ctggagcacc accctcaggg ccagcgggag ccctccggcc
      121 accggtatac aagtttgtac aaaaaagcag gctccgcggc catggcaggc ctgcagctca
      181 tgacccctgc ttcctcacca atgggtcctt tctttggact gccatggcaa caagaagcaa
      241 ttcatgataa catttatacg ccaagaaaat accaggttga gctgctggaa gcagctctgg
      301 accataacac aattgtgtgc ttgaacactg gctcagggaa gacgttcatc gcggtcctgc
      361 tcaccaaaga gctggcccat cagatcaggg gcgacctcaa cccgcatgca aaaaggaccg
      421 tgttcctcgt caactctgca aaccaggttg ctcaacaagt gtcagccgtc agaactcact
      481 cggatctcaa ggttggagag tactcagacc tagaagtaaa tgcatcttgg acaaaagaga
      541 gatggagcca agagtttact aagcaccagg ttctcattat gacttgctat gtcgccttga
      601 ctgttttgaa aaatggttac ttatcactgc cagacattaa ccttttggtg tttgatgagt
      661 gtcatcttgc gattctagac cacccctatc gtgaaattat gaagctctgt gagagttgtc
      721 catcatgccc tcgaattttg ggactaactg cttccatttt aaatgggaaa tgtgatccag
      781 aggaattgga aga
//
