LOCUS       sequence-77162-e       12168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: 
            Chloramphenicol Promoters: PLacUV5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, 
            Mukhopadhyay A, Petzold CJ, Keasling JD
  TITLE     Modular engineering of L-tyrosine production in Escherichia coli.
  JOURNAL   Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 
            10.1128/AEM.06017-11. Epub 2011 Oct 21.
  PUBMED    22020510
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.06017-11"; journalName: "Appl Environ Microbiol"; date:
            "2012-01"; volume: "78"; issue: "1"; pages: "89-98"
COMMENT     Sequence Label: pS3
FEATURES             Location/Qualifiers
     source          1..12168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      9838..9909
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9925..9952
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(10019..10681)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(11268..11362)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(11383..12042)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11957..11976
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(12043..12145)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atctaaagga ggccatccat ggaaacctat
     1621 gctgtttttg gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct
     1681 cagcaactga atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc
     1741 aacacactga acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt
     1801 aaagaagagg cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct
     1861 gttaataccc tcatgcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtgta
     1921 ggcttgttaa gcgatctgga acgtctgtct tttatccgcc ctggtttacg tattctgctt
     1981 atcggcgctg gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg
     2041 gtgacaataa ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac
     2101 actggcagta ttcaggcgtt gagtatggac gaactggaag gtcatgagtt tgatctcatt
     2161 attaatgcaa catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt
     2221 catccaggca tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca
     2281 tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag
     2341 gcggctcatg cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag
     2401 caattgcagg aggaattgtc cgcgtgagga tctaaaggag gccatccatg aaaaccgtaa
     2461 ctgtaaaaga cctggtcatt ggtacgggcg cacctaaaat catcgtctcg ctgatggcga
     2521 aagatatcgc cagcgtgaaa tccgaagctc tcgcctatcg tgaagcggac tttgatattc
     2581 tggaatggcg tgtggaccac tatgccgacc tctccaatgt ggagtctgtc atggcggcag
     2641 caaaaattct ccgtgagacc atgccagaaa aaccgctgct gtttaccttc cgcagtgcca
     2701 aagaaggcgg cgagcaggcg atttccaccg aggcttatat tgcactcaat cgtgcagcca
     2761 tcgacagcgg cctggttgat atgatcgatc tggagttatt taccggtgat gatcaggtta
     2821 aagaaaccgt cgcctacgcc cacgcgcatg atgtgaaagt agtcatgtcc aaccatgact
     2881 tccataaaac gccggaagcc gaagaaatca ttgcccgtct gcgcaaaatg caatccttcg
     2941 acgccgatat tcctaagatt gcgctgatgc cgcaaagtac cagcgatgtg ctgacgttgc
     3001 ttgccgcgac cctggagatg caggagcagt atgccgatcg tccaattatc acgatgtcga
     3061 tggcaaaaac tggcgtaatt tctcgtctgg ctggtgaagt atttggctcg gcggcaactt
     3121 ttggtgcggt aaaaaaagcg tctgcgccag ggcaaatctc ggtaaatgat ttgcgcacgg
     3181 tattaactat tttacaccag gcataaggat ctaaaggagg ccatccatgg agcgtattgt
     3241 cgttactctg ggcgaacgta gctacccaat taccatcgca tctggtttgt ttaatgaacc
     3301 agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca acgaaaccct
     3361 ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga
     3421 tagcgttatc ctccctgacg gcgagcagta taaaagcctg gctgtactcg ataccgtctt
     3481 tacggcgttg ttacaaaaac cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg
     3541 cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg tccgtttcat
     3601 tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc gttggcggca aaactgcggt
     3661 caaccatccc ctcggtaaaa acatgattgg cgcgttctac caacctgctt cagtggtggt
     3721 ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt
     3781 catcaaatac ggcattattc ttgacggtgc gttttttaac tggctggaag agaatctgga
     3841 tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt gttgtgaact
     3901 gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct
     3961 gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt ggttacatgg
     4021 tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt
     4081 tagttctgcc gaaacgcagc gtattataac cctgctcaag cgggctgggt taccggtcaa
     4141 tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt
     4201 ccttgcggga gagatgcgct taattcttcc gttggcaatt ggtaagagtg aagttcgcag
     4261 cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag cgtaaggatc
     4321 tggatctaaa ggaggccatc catgaattat cagaacgacg atttacgcat caaagaaatc
     4381 aaagagttac ttcctcctgt cgcattgctg gaaaaattcc ccgctactga aaatgccgcg
     4441 aatacggttg cccatgcccg aaaagcgatc cataagatcc tgaaaggtaa tgatgatcgc
     4501 ctgttggttg tgattggccc atgctcaatt catgatcctg tcgcggcaaa agagtatgcc
     4561 actcgcttgc tggcgctgcg tgaagagctg aaagatgagc tggaaatcgt aatgcgcgtc
     4621 tattttgaaa agccgcgtac cacggtgggc tggaaagggc tgattaacga tccgcatatg
     4681 gataatagct tccagatcaa cgacggtctg cgtatagccc gtaaattgct gcttgatatt
     4741 aacgacagcg gtctgccagc ggcaggtgag tttctcaaca tgatcacccc acaatatctc
     4801 gctgacctga tgagctgggg cgcaattggc gcacgtacca ccgaatcgca ggtgcaccgc
     4861 gaactggcat cagggctttc ttgtccggtc ggcttcaaaa atggcaccga cggtacgatt
     4921 aaagtggcta tcgatgccat taatgccgcc ggtgcgccgc actgcttcct gtccgtaacg
     4981 aaatgggggc attcggcgat tgtgaatacc agcggtaacg gcgattgcca tatcattctg
     5041 cgcggcggta aagagcctaa ctacagcgcg aagcacgttg ctgaagtgaa agaagggctg
     5101 aacaaagcag gcctgccagc acaggtgatg atcgatttca gccatgctaa ctcgtccaaa
     5161 caattcaaaa agcagatgga tgtttgtgct gacgtttgcc agcagattgc cggtggcgaa
     5221 aaggccatta ttggcgtgat ggtggaaagc catctggtgg aaggcaatca gagcctggag
     5281 agcggggagc cgctggccta cggtaagagc atcaccgatg cctgcatcgg ctgggaagat
     5341 accgatgctc tgttacgtca actggcgaat gcagtaaaag cgcgtcgcgg gtaaggatct
     5401 aaaggaggcc atccatgtcc aacaatggct cgtcaccgct ggtgctttgg tataaccaac
     5461 tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc ctccctgggt gaaatgatta
     5521 ctaatctttc cggaatgggt gtttccgttc cgaatggttt cgccacaacc gccgacgcgt
     5581 ttaaccagtt tctggaccaa agcggcgtaa accagcgcat ttatgaactg ctggataaaa
     5641 cggatattga cgatgttact cagcttgcga aagcgggcgc gcaaatccgc cagtggatta
     5701 tcgacactcc cttccagcct gagctggaaa acgccatccg cgaagcctat gcacagcttt
     5761 ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc cgccaccgca gaagatatgc
     5821 cggacgcttc ttttgccggt cagcaggaaa ccttcctcaa cgttcagggt tttgacgccg
     5881 ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cgatcgcgcc atctcttatc
     5941 gtgtgcacca gggttacgat caccgtggtg tggcgctctc cgccggtgtt caacggatgg
     6001 tgcgctctga cctcgcatca tctggcgtga tgttctccat tgataccgaa tccggctttg
     6061 accaggtggt gtttatcact tccgcatggg gccttggtga gatggtcgtg cagggtgcgg
     6121 ttaacccgga tgagttttac gtgcataaac cgacactggc ggcgaatcgc ccggctatcg
     6181 tgcgccgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc caggagcacg
     6241 gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc tcgctgacca
     6301 acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc
     6361 cgatggatat tgagtgggcg aaagatggcc acaccggtaa actgttcatt gtgcaggcgc
     6421 gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg cattcacagg
     6481 gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggtgcgggt ccggtgaaag
     6541 tcatccatga catcagcgaa atgaaccgca tcgaacctgg cgacgtgctg gttactgaca
     6601 tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatcgtc accaaccgtg
     6661 gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggcattccg gcggtagtgg
     6721 gctgtggaga tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg
     6781 aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa agctccagcg
     6841 tagaaacgat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac ccggaccgtg
     6901 ctttcgactt cgcctgccta ccgaacgaag gcgtgggcct tgcgcgtctg gaatttatca
     6961 tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat caggaaccgc
     7021 agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt gaattttacg
     7081 ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca
     7141 ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt
     7201 acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc tatgtttccg
     7261 acagcttccg cgactgtttc gcgctggagt gtgaagcagt gaaacgtgtg cgcaacgaca
     7321 tgggactgac caacgttgag atcatgatcc cgttcgtgcg taccgtagat caggcgaaag
     7381 cggtggttga agaactggcg cgtcaggggc tgaaacgtgg cgagaacggg ctgaaaatca
     7441 tcatgatgtg tgaaatcccg tccaacgcct tgctggccga gcagttcctc gaatatttcg
     7501 acggcttctc aattggctca aacgatatga cgcagctggc gctcggtctg gaccgtgact
     7561 ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc ggtgaaagca ctgctgtcga
     7621 tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt cagggtccgt
     7681 ccgaccacga agactttgcc gcatggttga tggaagaggg gatcgatagc ctgtctctga
     7741 acccggacac cgtggtgcaa acctggttaa gcctggctga actgaagaaa taaggatcta
     7801 aaggaggcca tccatgtcct cacgtaaaga gcttgccaat gctattcgtg cgctgagcat
     7861 ggacgcagta cagaaagcca aatccggtca cccgggtgcc cctatgggta tggctgacat
     7921 tgccgaagtc ctgtggcgtg atttcctgaa acacaacccg cagaatccgt cctgggctga
     7981 ccgtgaccgc ttcgtgctgt ccaacggcca cggctccatg ctgatctaca gcctgctgca
     8041 cctcaccggt tacgatctgc cgatggaaga actgaaaaac ttccgtcagc tgcactctaa
     8101 aactccgggt cacccggaag tgggttacac cgctggtgtg gaaaccacca ccggtccgct
     8161 gggtcagggt attgccaacg cagtcggtat ggcgattgca gaaaaaacgc tggcggcgca
     8221 gtttaaccgt ccgggccacg acattgtcga ccactacacc tacgccttca tgggcgacgg
     8281 ctgcatgatg gaaggcatct cccacgaagt ttgctctctg gcgggtacgc tgaagctggg
     8341 taaactgatt gcattctacg atgacaacgg tatttctatc gatggtcacg ttgaaggctg
     8401 gttcaccgac gacaccgcaa tgcgtttcga agcttacggc tggcacgtta ttcgcgacat
     8461 cgacggtcat gacgcggcat ctatcaaacg cgcagtagaa gaagcgcgcg cagtgactga
     8521 caaaccttcc ctgctgatgt gcaaaaccat catcggtttc ggttccccga acaaagccgg
     8581 tacccacgac tcccacggtg cgccgctggg cgacgctgaa attgccctga cccgcgaaca
     8641 actgggctgg aaatatgcgc cgttcgaaat cccgtctgaa atctatgctc agtgggatgc
     8701 gaaagaagca ggccaggcga aagaatccgc atggaacgag aaattcgctg cttacgcgaa
     8761 agcttatccg caggaagccg ctgaatttac ccgccgtatg aaaggcgaaa tgccgtctga
     8821 cttcgacgct aaagcgaaag agttcatcgc taaactgcag gctaatccgg cgaaaatcgc
     8881 cagccgtaaa gcgtctcaga atgctatcga agcgttcggt ccgctgttgc cggaattcct
     8941 cggcggttct gctgacctgg cgccgtctaa cctgaccctg tggtctggtt ctaaagcaat
     9001 caacgaagat gctgcgggta actacatcca ctacggtgtt cgcgagttcg gtatgaccgc
     9061 gattgctaac ggtatctccc tgcacggtgg cttcctgccg tacacctcca ccttcctgat
     9121 gttcgtggaa tacgcacgta acgccgtacg tatggctgcg ctgatgaaac agcgtcaggt
     9181 gatggtttac acccacgact ccatcggtct gggcgaagac ggcccgactc accagccggt
     9241 tgagcaggtc gcttctctgc gcgtaacccc gaacatgtct acatggcgtc cgtgtgacca
     9301 ggttgaatcc gcggtcgcgt ggaaatacgg tgttgagcgt caggacggcc cgaccgcact
     9361 gatcctctcc cgtcagaacc tggcgcagca ggaacgaact gaagagcaac tggcaaacat
     9421 cgcgcgcggt ggttatgtgc tgaaagactg cgccggtcag ccggaactga ttttcatcgc
     9481 taccggttca gaagttgaac tggctgttgc tgcctacgaa aaactgactg ccgaaggcgt
     9541 gaaagcgcgc gtggtgtcca tgccgtctac cgacgcattt gacaagcagg atgctgctta
     9601 ccgtgaatcc gtactgccga aagcggttac tgcacgcgtt gctgtagaag cgggtattgc
     9661 tgactactgg tacaagtatg ttggcctgaa cggtgctatc gtcggtatga ccaccttcgg
     9721 tgaatctgct ccggcagagc tgctgtttga agagttcggc ttcactgttg ataacgttgt
     9781 tgcgaaagca aaagaactgc tgtaaggatc caaactcgag taaggatctc caggcatcaa
     9841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     9901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc
     9961 taggctacag ccgatagtct ggaacagcgc acttacgggt tgctgcgcaa cccaagtgct
    10021 accggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg ctcgccatcg tccagaaaac
    10081 acggctcatc gggcatcggc aggcgctgct gcccgcgccg ttcccattcc tccgtttcgg
    10141 tcaaggctgg caggtctggt tccatgcccg gaatgccggg ctggctgggc ggctcctcgc
    10201 cggggccggt cggtagttgc tgctcgcccg gatacagggt cgggatgcgg cgcaggtcgc
    10261 catgccccaa cagcgattcg tcctggtcgt cgtgatcaac caccacggcg gcactgaaca
    10321 ccgacaggcg caactggtcg cggggctggc cccacgccac gcggtcattg accacgtagg
    10381 ccaacacggt gccggggccg ttgagcttca cgacggagat ccagcgctcg gccaccaagt
    10441 ccttgactgc gtattggacc gtccgcaaag aacgtccgat gagcttggaa agtgtcttct
    10501 ggctgaccac cacggcgttc tggtggccca tctgcgccac gaggtgatgc agcagcattg
    10561 ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg cgttccgttt
    10621 gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac tgcgtggcca
    10681 tgcttatctc catgcggtag gggtgccgca cggttgcggc accatgcgca atcagctgca
    10741 acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt
    10801 ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc
    10861 ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg
    10921 tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg
    10981 gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag
    11041 gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg
    11101 cgggcttcgc ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
    11161 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
    11221 cggcaagcga agcgcgcagg ccgcaggccg gaggcactag tgcttggatt ctcaccaata
    11281 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
    11341 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
    11401 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
    11461 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
    11521 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
    11581 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
    11641 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
    11701 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
    11761 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
    11821 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
    11881 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
    11941 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
    12001 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
    12061 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
    12121 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc cagatatc
//
