LOCUS       sequence-77184-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc01_3473
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             669..680
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttactgctg aatgctttcc agtgcaacac cggttgcatc atgataaaca
       61 tcctgctgca gctgattcag ctgacgtgca taaaacagct gatcatcact acgctgctga
      121 ttcacaaatt tgatcagttc tgcaaaatct tcacgacgtt ctttacggct gctgctcagc
      181 agataattgg tcagctggct tgcgctttcg ctctgctgct gctgcagggt ctgtgcatac
      241 agggtaaatg catcctgctg attctgttta aaggcatcca gtttgctatc aaccagttta
      301 accagtgcct gttcgctctg accgctacca aaacgaatac gcagttcacc ttcactggtg
      361 gtaacctggg tgccggtcag aaccagaaca attgccagtg cgctggtaac caggctaaca
      421 ccgctcagcc agctacctga attatttgcc tgctgcggtt caaaggttgc tgctttattc
      481 cactgcggaa cttcgctgct gctataatca tctgcaccaa ttttaaccat tgctgctgca
      541 tcaacctgtt ctgcaaactg gctatcctga atacaacgct gttcaaattc atcacgctgt
      601 ttcggggtca gtttaccttc cagccacagt gccagcagtt ctgcattatc atagttgctc
      661 atatggtatt tctcctcttt ctctagtatt tattcgacta taacaaacca ttttcttgcg
      721 taaacctgta cgatcctaca ggtcagccaa cgatcgttcc gatcagaaca agaaagctat
      781 gtggcctgac gaagcggcgc gccatcgaat ggcgcaaaac ctttcgcggt atggcatgat
      841 agcgcccgga agagagtcaa ttcagggtgg tgaatatgaa aaacataaat gccgacgaca
      901 catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct
      961 tatctga
//
