LOCUS       sequence-77214-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc25_1643_cl43
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             624..635
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttagttaat atcaggcgga atttcaccca gctgcggctg cggctggata
       61 aatttggtcg ggctggcaac tgctgctttc ggaatttcaa taaccggatt attcagtgca
      121 atcatcggca gtgcatccgg tgtggtaact gcctgggtcg gctgttcttg cggctgctgt
      181 gccagctgcg gaataccggt ctgacgacca ggcagccaat tggtaactgc accaataaca
      241 catgctgcaa ctgctgcacc acccagtttc catgccagac ggctacgacg attaactttt
      301 gcaaaaacct gttctgcggt ggtttccgga ctctgctgtg cggtcggaat cggcatggta
      361 cgaataccct gacgcagttt cagcagacgg ctatacagac gctgaactgc aacatcattt
      421 gccagccaat cttcaacctg acgacgttct gctgcggtaa cttcaccatc cagatatgcg
      481 ctcagcagtt cagaacgatc acgtttaacc atatccattg caccggttgc ttcattggta
      541 tgacgtgcca ttttctgcgg cagatcacgg ctaaactgac gacgacacgg atcattaaag
      601 ttgctatcgg tggtcatatg gtatttctcc tctttctcta gtatttattc gactataaca
      661 aaccattttc ttgcgtaaac ctgtacgatc ctacaggtca gccaacgatc gttccgatca
      721 gaacaagaaa gctatgtggc ctgacgaagc ggcgcgccat cgaatggcgc aaaacctttc
      781 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat atgaaaaaca
      841 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
      901 atattaatca atgcttatct ga
//
