LOCUS       sequence-77222-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Blast DEST (w46-2)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      34..169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    209..330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(220..239)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     274..293
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             402..582
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    657..781
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gactcactat agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact
       61 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
      121 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
      181 ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca
      241 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      301 gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc
      361 aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac
      421 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
      481 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
      541 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag
      601 atcttgtctt cgttgggagt gagctagtcc agtgtggtgg aattctgcag atatcaacaa
      661 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      721 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      781 ggcggccgca tta
//
