LOCUS       sequence-77647-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50014
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             227..253
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             263..286
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(584..602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
       61 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      181 tctccccgcg cgttggccga ttcattaatc actgtggtct cattcgtacc cttatgacgt
      241 tcctgattat gcatccggtg gcctcgaagt tttgttccag ggtccactgg gttccgcaga
      301 tccaaccagc tccgaaacat catcacaccc tccaggaacc attgtggccc ccatggctgg
      361 tttggtagtc aaggtccttg tcgaaaacga agccaaagta gatcaaggtc aacctatatt
      421 agtcctagag gcaatgaaga tggagcacgt tgtgaaagca ccatcctctg gaagcataca
      481 ggacctcaaa gttaaagcag gccaacaggt ttcagatggc agtgctctat tcaggatcaa
      541 agggtaagct ttgagaccac gtggctcttc agtggacgaa agggcctcgt gatacgccta
      601 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      721 ctcatgagac aataaccctg ataaatgctt caataatatt
//
