LOCUS       sequence-77671-e        6145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, galactose inducible, 
            URA3 selection .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-GU
FEATURES             Location/Qualifiers
     source          1..6145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        624..1288
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    840..957
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1252..1275
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      1325..1624
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      1649..2993
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3104..3907)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3908..4128)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4153..4171)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4239..4343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4344..4412,4413..5204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4562..4581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5375..5963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5864..5883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6117..6134
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc ccctttcaaa aattcttact ttttttttgg atggacgcaa
      661 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc
      721 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa
      781 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
      841 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
      901 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
      961 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     1021 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     1081 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     1141 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     1201 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     1261 ctatacttta acgtcaagga gaaaaaaccc gggtaccgag ctcgaattcg acacttctaa
     1321 ataagcggat ctcctatgcc ttcacgattt atagtttcca ttatcaagta tgcctatatt
     1381 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta
     1441 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt
     1501 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc
     1561 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc
     1621 tcattttcct tcgcatgccc tcgagcggaa cgaagcatct gtgcttcatt ttgtagaaca
     1681 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     1741 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     1801 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     1861 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     1921 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     1981 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     2041 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     2101 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     2161 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     2221 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     2281 tatattacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     2341 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     2401 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     2461 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     2521 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     2581 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag
     2641 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
     2701 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
     2761 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
     2821 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
     2881 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
     2941 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcgatccga
     3001 tgataagctg tcaaacatga gaattgggta ataactgata taattaaatt gaagctctaa
     3061 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     3121 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     3181 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     3241 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     3301 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     3361 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     3421 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     3481 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     3541 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     3601 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     3661 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     3721 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     3781 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     3841 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     3901 tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg gttaagaata
     3961 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     4021 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaagaa
     4081 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa gctaattctt
     4141 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4201 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4261 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4321 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4381 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4441 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4501 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4561 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4621 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4681 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4741 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4801 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4861 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4921 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4981 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5041 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5101 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5161 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5221 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5281 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5461 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5521 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5581 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5641 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5701 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5761 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5821 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5881 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5941 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6001 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6061 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6121 agtcagtgag cgaggaagcg gaaga
//
