LOCUS       sequence-77673-e        5893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, galactose inducible, 
            TRP1 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-GT
FEATURES             Location/Qualifiers
     source          1..5893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        624..1288
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    840..957
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1252..1275
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      1325..1624
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      1649..2993
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3105..3779)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3780..3881)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(3901..3919)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3987..4091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4092..4160,4161..4952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4310..4329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5123..5711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5612..5631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5865..5882
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc ccctttcaaa aattcttact ttttttttgg atggacgcaa
      661 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc
      721 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa
      781 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
      841 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
      901 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
      961 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     1021 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     1081 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     1141 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     1201 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     1261 ctatacttta acgtcaagga gaaaaaaccc gggtaccgag ctcgaattcg acacttctaa
     1321 ataagcggat ctcctatgcc ttcacgattt atagtttcca ttatcaagta tgcctatatt
     1381 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta
     1441 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt
     1501 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc
     1561 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc
     1621 tcattttcct tcgcatgccc tcgagcggaa cgaagcatct gtgcttcatt ttgtagaaca
     1681 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     1741 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     1801 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     1861 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     1921 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     1981 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     2041 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     2101 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     2161 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     2221 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     2281 tatattacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     2341 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     2401 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     2461 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     2521 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     2581 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag
     2641 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
     2701 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
     2761 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
     2821 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
     2881 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
     2941 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcgatccga
     3001 tgataagctg tcaaacatga gaattgatct tttatgcttg cttttcaaaa ggcttgcagg
     3061 caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt
     3121 ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt
     3181 acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt
     3241 ccaccagcta acataaaatg taagctctcg gggctctctt gccttccaac ccagtcagaa
     3301 atcgagttcc aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa caagggaata
     3361 aacgaatgag gtttctgtga agctgcactg agtagtatgt tgcagtcttt tggaaatacg
     3421 agtcttttaa taactggcaa accgaggaac tcttggtatt cttgccacga ctcatctcca
     3481 tgcagttgga cgatatcaat gccgtaatca ttgaccagag ccaaaacatc ctccttaggt
     3541 tgattacgaa acacgccaac caagtatttc ggagtgcctg aactattttt atatgctttt
     3601 acaagacttg aaattttcct tgcaataacc gggtcaattg ttctctttct attgggcaca
     3661 catataatac ccagcaagtc agcatcggaa tctagtgcac attctgcggc ctctgtgctc
     3721 tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat aacagacata
     3781 ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc
     3841 cctccctctt ggccctctcc ttttcttttt tcgaccgaat taattcttga agacgaaagg
     3901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5881 aggaagcgga aga
//
