LOCUS       sequence-77737-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) Ef1a
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(35..246)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ttaatcagct cgctagccat ggtggcggca gatcctgtgt tctggcggca aacccgttgc
       61 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
      121 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
      181 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
      241 ctgcccactg acgggcaccg gagcctgaat tccggaaaca cccgaatcaa cttctagtca
      301 aattattgtt cacgccgcaa tgacccaccc ctggcccgcg tctgtggaac tgacccctgg
      361 tgtacaggag agttcgctgc tgaaagtggt cccaaagggg tactagtttt taagctccca
      421 actccccctc ccccagcgtc tggaggattc cacaccctcg caccgcaggg gcgaggaagt
      481 gggcggagtc cggttttggc gccagccgct gaggctgcca agcagaaaag ccaccgctga
      541 ggagactccg gtcactgtcc tcgccccgcc tcccccttcc ctccccttgg ggaccaccgg
      601 gcgccacgcc gcgaacggta agtgccgcgg tcgtcggcgc ctccgccctc
//
