LOCUS       sequence-77779-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of a catalytically inactive bacterial Cas9 
            (S. pyogenes) for bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pdCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacctccacg ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt
       61 ttagaagttg tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat
      121 ccgatttacc acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt
      181 taaggaaact ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta
      241 attcttggtc cacatacatg tctcttccat tttggagata atagagatag agcttttcat
      301 tttgcaattg agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga
      361 taccttcttc gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct
      421 gattttcacg tgccatttca ataacgatat tttctggctt atgccgcccc attactttga
      481 ccaattcatc aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag
      541 ctaaatttgc aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa
      601 tgtcttcttt aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa
      661 aaccatctga tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat
      721 taatcaattt tcgagacaaa cgtccccaac cagtataacg gcgacgttta agctgtttca
      781 tcaccttatc atcaaagagg tgagcatatg ttttaagtct ttcctcaatc atctccctat
      841 cttcaaataa ggtcaatgtt aaaacaatat cctctaagat atcttcattt tcttcattat
      901 ccaaaaaatc tttatcttta ataattttta gcaaatcatg gtaggtacct aatgaagcat
      961 taaatctatc ttcaactcc
//
