LOCUS       sequence-7780-ex        8280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Bateman JR, Lee AM, Wu CT
  TITLE     Site-specific transformation of Drosophila via phiC31 
            integrase-mediated cassette exchange.
  JOURNAL   Genetics. 2006 Jun . 173(2):769-77.
  PUBMED    16547094
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2006 Jun . 173(2):769-77."
COMMENT     Sequence Label: pUASTP2.1
FEATURES             Location/Qualifiers
     source          1..8280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     protein_bind    776..875
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             complement(join(1590..2204,2275..2406,2627..2942,3004..3658,
                     3733..4006,4401..4472))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4924..4942
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    5040..5139
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     misc_feature    join(5288..5489,5490..5520)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(6057..6076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6176..6198
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6236..6254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6322..6426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6427..6495,6496..7287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6645..6664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7947..7966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca gtactgacgg
      721 acacaccgaa gccccggcgg caaccctcag cggatgcccc ggggcttcac gttttcccag
      781 gtcagaagcg gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt
      841 gggggcgtag ggtcgccgac atgacacaag gggttgtgac cggggtggac acgtacgcgg
      901 gtgcttacga ccgtcagtcg cgcgagcgcg actgcatcta cacaaggaac aaacactgga
      961 tgtcactttc agttcaaatt gtaacgctaa tcactccgaa caggtcacaa aaaattacct
     1021 taaaaagtca taatattaaa ttagaataaa tatagctgtg agggaaatat atacaaatat
     1081 attggagcaa ataaattgta catacaaata tttattacta atttctattg agacgaaatg
     1141 aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag
     1201 gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac
     1261 tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt
     1321 atataaaaaa tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat
     1381 gcaggtgtgt aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg
     1441 aaggggaaat acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta
     1501 cattagcttt ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa
     1561 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     1621 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagacccac gtagtccagc
     1681 ggcagatcgg cggcggagaa gttaagcgtc tccaggatga ccttgcccga actggggcac
     1741 gtggtgttcg acgatgtgca gctaatttcg cccggctcca cgtccgccca ttggttaatc
     1801 agcagaccct cgttggcgta acggaaccat gagaggtacg acaaccattt gaggtatact
     1861 ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca ggaatggtat gataaccggc
     1921 ggacccacag acagcgccat cgaggtcgag gagctggcgc aggatattag atatccgaag
     1981 gacgttgaca cattggccac cagagtgacc agcgccaggc agttgaagaa gtgcagcact
     2041 ccggcccgca gtccgatcat cggataggca atcgccgtga agaccagtgg cactgtgaga
     2101 aaaagcggta attcggcaat cgttttgccc agaaagtatg tgtcacagcg ataaagtcga
     2161 cttcgggcct ccctcataaa aactggcagc tctgaggtga acacctaaat cgaatcgatt
     2221 cattagaaag ttagtaaatt attaatatgc aaatgtattc taaacaagac ttacatttat
     2281 cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag aggaagatgg ctccgttgat
     2341 attcatcacg cccacttgcg tgagttgttg gcccaaaaag atgaggccaa tcaagatggc
     2401 aaccatctgc aaattaaaat gttactcgca tctcattaat attcatatct tcaacatgtt
     2461 cgcgagttaa atgaaattta tttattttct gcaaaactat aaactataca tctcattgaa
     2521 aaaaactaag aagggtgtgg aatcaggcaa ttctaactaa aatctagcga atttgtttcc
     2581 aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt gtctgaataa
     2641 gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc cacaggaccg
     2701 cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc tgctccagtg
     2761 gcttctccaa atttttggtg gccaacaact gctccatatc ccgggctact ttgctaatag
     2821 caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt ccgggcacaa
     2881 cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac tgggcaccca
     2941 cgctggatag gagttgagat gtaatgtaat gctagatacc cttaataaac acatcgaact
     3001 cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac cctgccctcg
     3061 gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg atgaatggtc
     3121 aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac gctgtgggcg
     3181 gtaaatgagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg atcggttagt
     3241 gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt caccctgccg
     3301 ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat cacctgatcc
     3361 acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat ggcctggaaa
     3421 atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg gacataggcg
     3481 cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg catcccggat
     3541 ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt
     3601 ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac gccgcaaact
     3661 gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag ccgactgcga
     3721 atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc
     3781 agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc tgattgaccg
     3841 ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt tggccgctcc
     3901 ctgaaccgga gtcctccggc ggactgggtg gcaggagcgt gccgtagttt ttggcctgcc
     3961 cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc aatgataggg
     4021 gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa cgaagtatcc
     4081 tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc ttaaaatatc
     4141 tggttgttat attttttgta aaaaagaatg tagtcgaaaa tgaatgcctt tagatgtctt
     4201 gatcatgata tgatcttaaa aattgtctta tatagcgagc acagctacca gaataatctg
     4261 tttcgtgtca ctatttgttt gtgcgattgc ggtttgggat ttttgtgggt cgcagttctc
     4321 acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga acttaagatg
     4381 tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt gctgcctccg
     4441 cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac tttcccctgc
     4501 ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa aataagaagc
     4561 gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc ttggagcttc
     4621 gtcacgaaac cgctgacaaa gtgcaactga aggcggacat tgacgctagg taacgctaca
     4681 aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat ttctgtggac
     4741 agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg agaggtaatc
     4801 gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact gatcagataa
     4861 gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac tttccgcaaa
     4921 aatgggtttt attaacttac atacatacta gaattaggat ctgcagaatt cggcttgtac
     4981 tagtcgcgct cgcgcgactg acggtcgtaa gcacccgcgt acgtgtccac cccggtcaca
     5041 accccttgtg tcatgtcggc gaccctacgc ccccaactga gagaactcaa aggttacccc
     5101 agttggggca ctactcccga aaaccgcttc tgacctggga aaacgtgaag ccccggggca
     5161 tccgctgagg gttgccgccg gggcttcggt gtgtccgtca gtactagtcg aagccgaatt
     5221 ccagcacact ggcggccgtt actagtggat ccggagagct cccaacgcgt tggatgcagc
     5281 caagctttgc gtactcgcaa attattaaaa ataaaacttt aaaaataatt tcgtctaatt
     5341 aatattatga gttaattcaa accccacgga catgctaagg gttaatcaac aatcatatcg
     5401 ctgtctcact cagactcaat acgacactca gaatactatt cctttcactc gcacttattg
     5461 caagcatacg ttaagtggat gtctcttgcc gacgggacca ccttatgtta tttcatcatg
     5521 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     5581 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     5641 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     5701 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     5761 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     5821 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     5881 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     5941 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     6001 cgttagttcc gcgcgattcg gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     6061 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     6121 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6181 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     6241 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6661 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8041 tggaaaaacg ccttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
     8101 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
     8161 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
     8221 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
//
