LOCUS       sequence-7783-ex        9458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Bateman JR, Lee AM, Wu CT
  TITLE     Site-specific transformation of Drosophila via phiC31 
            integrase-mediated cassette exchange.
  JOURNAL   Genetics. 2006 Jun . 173(2):769-77.
  PUBMED    16547094
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2006 Jun . 173(2):769-77."
COMMENT     Sequence Label: pCiB-yin
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    65..134
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             4060..4071
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5537..5553)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5537..5553)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    5776..5845
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(5880..6434,6435..6465)
                     /label=P element 5' end
     rep_origin      complement(6700..7288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6780..6799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7459..8250,8251..8319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8082..8101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8320..8424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8492..8510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8548..8570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8670..8689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9226..9256,9257..9458)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttgggc tgcaggtcga ctctagagcg taccgggccc cccctcgacg atgtaggtca
       61 cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
      121 ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg
      181 cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg
      241 tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt
      301 tctcgacggt cacggcgggc atgtcgaggt cgactattaa atgattatcg cccgattacc
      361 acattgagtg gtttaaaata gccataaaat atgcaactga cgatggctta agataaatac
      421 gtcgcagagt cactcataaa tttcgaacgc agcccgctga tttacctacc cctctaaacg
      481 attcatagta tatgtacgag tatatccact aagctttttc gagcactgat tttttcgctt
      541 gcacgagaca agtgcaccac cgcaattgca ggcaaattat gtctgaggta atgattccgt
      601 ttcgtgcaag attacacaga aatcaaatta cgacaacctt tattcagtaa gcaaacaaag
      661 cctttgttgg catctaatta ttccacttat ggttgcgatt tcgggagcta caatcggttt
      721 tggtttagta tatctagcga gttccttggc gacatttaaa atttacaaat aaagtttctc
      781 tattcaatcg gaacagtgga aattgactat tttatttata ttaatgaact tatttttaat
      841 ttggcttaag ttactaaggg gtactaatag tttgagcgca gtgcatgtca tggggacatg
      901 tgcaattgtg tgtaaacggg aagtgatcgc ggccttccga atttggccat gccaaataat
      961 cccagctcga aaggagggga cccggcggtc agggccatgg acattgaact tgaaaaaaaa
     1021 aaaacacaaa aatatataac acaaaacgga aaatgctgtg taccgcttat gttagagaag
     1081 ttgagcaacg ggtttttcgt tttgcagtca cgatggattt ccaaattagt gtaggagggg
     1141 ggaggggagg gagggagata atgtccaggc tgccataagt ggggaataag gaaaataaaa
     1201 catgaaacac gggtcgggca atgtcatgcg gtattcggct ttgctttccg cccaagttga
     1261 agtgatcctg tgtgtaaata atgtcgaatg ttgccggtcg gttgcataag cgttggtcaa
     1321 ttatggccaa agagatctga tttgtggaag ctttttttga ccacttagcg cgctccgctg
     1381 atgttgtttt gttttgtgct ggggcagaaa acttgtttca attattggga aaagtgcgta
     1441 taaatcattg ccgcaagctc tgaaaagcga aaaagaaaaa cagtaaccaa acagacaaac
     1501 gcagcattcc cccacacaat taagcaaaaa cttgaaacaa gtcaattcga aaaaaattat
     1561 aggttcaacg gctgcagcga tcgcatcatt agttgcgttt ttagtaaata caccatttca
     1621 ttacacaaca cacacaatta attaataaaa ctgtactggt tatttcaagt gtgtctttta
     1681 ataagcctgc cgatcgcaat aaattcgagc agcattgccg gtaattttgt gcaacatatt
     1741 tttcgattgc cacaccgtgt ttgtttattt tttctgtggg tgcaatgatt tagaatgcgg
     1801 gcaagggatc aagttgaacc acttctaaga aaaaatagac attgcataaa tgatatagag
     1861 tccaaaaact acaccaattc aatagcagta atggttacat tagctttgaa attgttttta
     1921 gacatccgaa gaaataagat taaatttaaa cggcattctt taatttgtat tttaatattt
     1981 tgagaggttt tccttattta aagtgtagat tattgaggat taatgcaata ccactttacc
     2041 tgcggaggtc gtaaaacgta tttttaccca tttgcatgtt tattatgcgt gtcgctggtt
     2101 gtttacttta cttaagtttt gcaatttttt ctttagcaag caggtgcatt tgggccaaga
     2161 gatatatgcg atcgctttcg gttcgaattt ttaacattta cttgcggcga tggtcattag
     2221 agcattaccc acttagggca cccccaacat ccagttgatt ttcagggacc acaatatttt
     2281 aaataacagc tagtggaatt acctaaaagc gctttcgtcc cttttgaaat tttatgtaac
     2341 actcaattat atttatgtat atgtatgctc aaaatcacct gccaataact agcggaaacc
     2401 aaatatttga ccctcagtga attgtgaatc atcggtgacg cccaatcgaa atccaatcct
     2461 aagcaattga aacgagcacg agttccaatt taatagtata caaggaaaca cctgctttaa
     2521 atactctaca tagtacacgt tataataacg atttatttga tatttctgga tttttgtctg
     2581 catgtatttc atataatatt gatttgattt ttttaatgaa ttgaactaaa aaatcatatt
     2641 agaacatttt ttgcagtcgc cgataaagat gaacactgtt ctcagaacac aactgtcatg
     2701 tattaagctt tcagattttc agaaatttgg agagcaatgc attctatgca cgagcctcct
     2761 ggccttacaa tttacttgtt tgaaattaga tcgtcaaata aagtccctaa aattaaataa
     2821 atagtagtca caactttaaa ataggtctta atcttttagg gtaccgaaag gtatttcggc
     2881 acaaatcagc gcagttttaa atgtcgatga aggccaaaaa tcataccaaa cccagcgaaa
     2941 ggtgatgtct gactcattaa attgggggat tcgagtgtat ttattaaaca tgcgtgaaaa
     3001 tcaatcatgg aagacaaaac gcaaagttgg ccgatctatg ggaacagcat aagccacctg
     3061 attacccgaa cactgaacca cccgaatcac taaaaccacc gaagttggcg cgcgccttcg
     3121 ttttcatttt cattggcctg tcttcgtctt cggagaaaaa aaccttcata taaaacgcgg
     3181 ccgacatatt atggccacca gtcgttaccg cgccacggtc cacagaagag gattaaaaaa
     3241 atatcacaca gccgaaggct agagaagaac cccctatagc tgaacatata taaacaaata
     3301 tatttttttt tattgccaac acactttggc ttaagtgtta agagtgattg tcagcttaga
     3361 gctaagtgca atgttccagg acaaagggtg gatccttgtg accctgatca ccttggtgac
     3421 gccgtcttgg gctgcttaca aacttcagga gcgatatagt tggagccagc tggactttgc
     3481 tttcccgaat acccgactaa aggaccaagc tctggctagt ggagattata ttccgcaaaa
     3541 tgctctacct gttggagtcg aacactttgg caatcggtta ttcgtcactg ttccccgctg
     3601 gcgtgatggg attccggcca ctctgaccta tataaacatg gaccgcagtt tgacgggttc
     3661 accggagcta attccgtatc cagattggcg ctcaaataca gctggagatt gcgccaacag
     3721 tattaccact gcctaccgca ttaaagtgga tgagtgtggt cggctgtggg ttttggacac
     3781 tggaaccgtg ggcatcggca ataccaccac taatccgtgc ccctatgcgg taaatgtctt
     3841 tgacttgacc acggatacgc gaattcggag atacgagcta cctggcgtgg acacaaatcc
     3901 aaatactttc atagctaaca ttgccgtgga tataggcaaa aattgcgatg atgcatatgc
     3961 ctattttgcc gatgaattgg gatacggctt gattgcttac tcctgggaac tgaacaagtc
     4021 ctggagattc tcggcacatt cgtatttttt ccccgatcca ttgaggggcg atttcaatgt
     4081 cgctggtatt aacttccaat ggggcgagga gggtatattt ggtatgtccc tttcgcccat
     4141 tcgatcggat ggttatcgta ccctgtactt tagtccgtta gcaagtcatc gacaatttgc
     4201 cgtatccacg aggattttga gggatgaaac caggacggaa gatagctatc atgactttgt
     4261 tgccttagat gaacggggtc caaactccca taccacttca cgtgtgatga gcgatgatgg
     4321 aattgagctg ttcaatttaa tagatcaaaa tgcagtgggt tgctggcact catcaatgcc
     4381 gtactcaccg caatttcatg gcattgtgga tcgcgatgac gttggcttag tttttccggc
     4441 cgatgtgaaa attgatgaga acaaaaacgt ttgggttcta tccgatagga tgcccgtttt
     4501 cttgctgtct gacttggatt attcagatac taatttccga atttacacgg ctcccttggc
     4561 cactttaatt gagaatactg tgtgtgattt gaggaataac gcctatgggc cgccaaatac
     4621 cgtttcaata ccaaaacaag ccgttttgcc aatgggtcca ccgttatata cgaaacaata
     4681 tcgtcctgtc ttgccacaga aacctcagac cagctgggct tcctcgccgc ctcctccaag
     4741 tcgcacttat ttgcccgcca attcaggcaa tgtagtctcc agtattagtg tctctacaaa
     4801 ttctgtgggt cctgcaggag tggaggtgcc aaaggcctat attttcaacc agcacaacgg
     4861 cataaattac gagacaagtg gtccccatct atttcccacc catcaacccg cccaaccggg
     4921 tggccaggat ggtgggttaa aaacttatgt gaatgcccgc caatctgggt ggtggcatca
     4981 tcagcatcaa ggttaacata atcctacaca cggtacttgg gtatattctc acacactcga
     5041 ttgatgtaaa gaatatttaa agacaacaac atagggcaac agcggttaaa aaaaccacat
     5101 gacgtatgag caagtggcaa atcaatactt tatctagtta tgttaagcaa aaaataacaa
     5161 taaatcaact tttttttgaa ggttaagagt ttacgcaatt ttcttgagcg gaaaaagcgg
     5221 aaaaaatgta agtatgcata aattctaaat atatcaacaa ctgtacattt tctggagtac
     5281 tactaccagg caagaaagta ggttgataaa gctatgcaca agatcttgtt tgggtgcagg
     5341 gaaagttcaa cttaatcgct caatttgaga tcgcctggtc gcttgagatt cgactgtaat
     5401 tgaaattttt gcttttgatc ggagccagac ttcagacggg gcaaacaaaa agactttgtt
     5461 ggtggtaggg taggatccgt tgacctgcan gtcgacggta tcgataagct tgatatcgaa
     5521 ttcctgcagc ccgggggatc cactagttct agaggatccc cgggcgagct cgaattcgcc
     5581 cttctgcaga tgccgcgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc
     5641 cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg
     5701 cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt
     5761 catcatgatg gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc
     5821 ctggcacccg caccgcggct tcgagaccgt gacctacatc ctgcagaagg gcgaattcgg
     5881 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
     5941 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
     6001 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
     6061 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
     6121 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta aagtgggtat
     6181 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
     6241 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
     6301 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
     6361 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
     6421 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta
     6481 gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact
     6541 ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg
     6601 gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag
     6661 gtatactggc accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc
     6721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     6901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7141 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8041 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     8101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8701 ccatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     8761 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     8821 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     8881 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     8941 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     9001 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     9061 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     9121 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     9181 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     9241 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     9301 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     9361 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     9421 tttttaaagt tttattttta ataatttgcg agtacgca
//
