LOCUS       sequence-77896-e        7329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the Dre-dependent Roxed-Cre gene under the control of 
            the human GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: hGFAP-Roxed-Cre
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2298..2318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2443..2474
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    2662..2796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2916..3050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3170..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3333..3364
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             4217..4240
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4263..4280)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4269..4493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4661..4678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4832..5420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4912..4931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5591..6382,6383..6451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6452..6556)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6583..7038
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6670..6689)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6880..6901
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7169..7217
                     /note="synthetic polyadenylation signal"
     misc_feature    7231..7322
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7271..7290
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgagctccca cctccctctc
       61 tgtgctggga ctcacagagg gagacctcag gaggcagtct gtccatcaca tgtccaaatg
      121 cagagcatac cctgggctgg gcgcagtggc gcacaactgt aattccagca ctttgggagg
      181 ctgatgtgga aggatcactt gagcccagaa gttctagacc agcctgggca acatggcaag
      241 accctatctc tacaaaaaaa gttaaaaaat cagccacgtg tggtgacaca cacctgtagt
      301 cccagctatt caggaggctg aggtgagggg atcacttaag gctgggaggt tgaggctgca
      361 gtgagtcgtg gttgcgccac tgcactccag cctgggcaac agtgagaccc tgtctcaaaa
      421 gacaaaaaaa aaaaaaaaaa aaaaaagaac atatcctggt gtggagtagg ggacgctgct
      481 ctgacagagg ctcgggggcc tgagctggct ctgtgagctg gggaggaggc agacagccag
      541 gccttgtctg caagcagacc tggcagcatt gggctggccg ccccccaggg cctcctcttc
      601 atgcccagtg aatgactcac cttggcacag acacaatgtt cggggtgggc acagtgcctg
      661 cttcccgccg caccccagcc cccctcaaat gccttccgag aagcccattg agcagggggc
      721 ttgcattgca ccccagcctg acagcctggc atcttgggat aaaagcagca cagcccccta
      781 ggggctgccc ttgctgtgtg gcgccaccgg cggtggagaa caaggctcta ttcagcctgt
      841 gcccaggaaa ggggatcagg ggatgcccag gcatggacag tgggtggcag ggggggagag
      901 gagggctgtc tgcttcccag aagtccaagg acacaaatgg gtgaggggac tgggcagggt
      961 tctgaccctg tgggaccaga gtggagggcg tagatggacc tgaagtctcc agggacaaca
     1021 gggcccaggt ctcaggctcc tagttgggcc cagtggctcc agcgtttcca aacccatcca
     1081 tccccagagg ttcttcccat ctctccaggc tgatgtgtgg gaactcgagg aaataaatct
     1141 ccagtgggag acggaggggt ggccagggaa acggggcgct gcaggaataa agacgagcca
     1201 gcacagccag ctcatgtgta acggctttgt ggagctgtca aggcctggtc tctgggagag
     1261 aggcacaggg aggccagaca aggaaggggt gacctggagg gacagatcca ggggctaaag
     1321 tcctgataag gcaagagagt gccggccccc tcttgcccta tcaggacctc cactgccaca
     1381 tagaggccat gattgaccct tagacaaagg gctggtgtcc aatcccagcc cccagcccca
     1441 gaactccagg gaatgaatgg gcagagagca ggaatgtggg acatctgtgt tcaagggaag
     1501 gactccagga gtctgctggg aatgaggcct agtaggaaat gaggtggccc ttgagggtac
     1561 agaacaggtt cattcttcgc caaattccca gcaccttgca ggcacttaca gctgagtgag
     1621 ataatgcctg ggttatgaaa tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag
     1681 cccttccttc cctttttttt tttttttttt gtgagacaag gtctctctct gttgcccagg
     1741 ctggagtggc gcaaacacag ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc
     1801 agcctcagcc tcccaaagtg ctgggattac aagcatgagc caccccactc agccctttcc
     1861 ttccttttta attgatgcat aataattgta agtattcatc atggtccaac caaccctttc
     1921 ttgacccacc ttcctagaga gagggtcctc ttgcttcagc ggtcagggcc ccagacccat
     1981 ggtctggctc caggtaccac ctgcctcatg caggagttgg cgtgcccagg aagctctgcc
     2041 tctgggcaca gtgacctcag tggggtgagg ggagctctcc ccatagctgg gctgcggccc
     2101 aaccccaccc cctcaggcta tgccaggggg tgttgccagg ggcacccggg catcgccagt
     2161 ctagcccact ccttcataaa gccctcgcat cccaggagcg agcagagcca gagcaggttg
     2221 gagaggagac gcatcacctc cgctgctcgc ggggatctgc gatctaagta agcttgaatt
     2281 ctcgagccgc caccatgcct aagaagaaga ggaaggtgac ctctgatgaa gtcaggaaga
     2341 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     2401 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa cgtaacttta aataattggc
     2461 attatttaaa gttaatgtat gcgctacgaa gttattcgcg atgaataaat gaaagcttgc
     2521 agatctgcga ctctagagga tctgcgactc tagaggatca taatcagcca taccacattt
     2581 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2641 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     2701 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2761 tccaaactca tcaatgtatc ttatcatgtc tggatctgcg actctagagg atcataatca
     2821 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2881 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta
     3061 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3121 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3301 gatccccatc aagctgatcc ggaaccctta attaacttta aataattggc attatttaaa
     3361 gttaaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc tcctgtacct
     3421 gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc tcaacatgct
     3481 gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc tggtgatgag
     3541 gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc tggcctttga
     3601 acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat gccaggacat
     3661 caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg ccgaaattgc
     3721 cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga tccacattgg
     3781 caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc tgggggttac
     3841 caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca acaactacct
     3901 gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc aactgtccac
     3961 ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga
     4021 ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaggga
     4081 catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt
     4141 gaacattgtg atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct
     4201 gctcgaggat ggggacgatt acaaggatga cgacgataag taggtcagag ctcgctgatc
     4261 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4321 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4381 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4441 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4501 ggcggaaaga accagctggg gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     4561 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     4621 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc
     4681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     5041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5461 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5521 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5581 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5641 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5701 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5761 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5821 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5881 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5941 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6001 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6061 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6121 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6181 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6241 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6301 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6361 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6421 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6481 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6541 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     6601 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6661 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6721 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6781 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6841 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6901 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6961 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7021 tattaacgtt tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7081 cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta
     7141 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg
     7201 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     7261 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     7321 ctatcgata
//
