LOCUS       sequence-77942-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity
            control FRET sensor for MagFRET-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M
  TITLE     MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor.
  JOURNAL   PLoS One. 2013 Dec 2;8(12):e82009. doi: 
            10.1371/journal.pone.0082009.
  PUBMED    24312622
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-2"; volume: "8(12):e82009. doi"; pages: " 
            10.1371/journal.pone.0082009"
COMMENT     Sequence Label: pCMVMagFRET-6-NLS
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(42..44,45..47,48..560)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(348..367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccgcca ccatgggcca tatggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggtaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      241 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
      481 tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      541 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      661 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactagcgag gaacagaaac aagaaattaa agatgctttt gaactgtttg
      781 atacagacaa agaagaagaa atagattatc atgaactgaa ggtggcaatg agagccttgg
      841 ggtttgatgt aa
//
